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首页> 外文期刊>International Journal of Tissue Engineering >The Use of a Green Fluorescent Protein Porcine Model to Evaluate Host Tissue Integration into Extracellular Matrix Derived Bionanocomposite Scaffolds
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The Use of a Green Fluorescent Protein Porcine Model to Evaluate Host Tissue Integration into Extracellular Matrix Derived Bionanocomposite Scaffolds

机译：使用绿色荧光蛋白猪模型评估宿主组织整合入细胞外基质衍生的Bionanocomposite支架。

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When using heterogeneous extracellular matrix (ECM) derived scaffolds for soft tissue repair, current methods ofin vivoevaluation can fail to provide a clear distinction between host collagen and implanted scaffolds making it difficult to assess host tissue integration and remodeling. The purpose of this study is both to evaluate novel scaffolds conjugated with nanoparticles for host tissue integration and biocompatibility and to assess green fluorescent protein (GFP) expressing swine as a new animal model to evaluate soft tissue repair materials. Human-derived graft materials conjugated with nanoparticles were subcutaneously implanted into GFP expressing swine to be evaluated for biocompatibility and tissue integration through histological scoring and confocal imaging. Histological scoring indicates biocompatibility and remodeling of the scaffolds with and without nanoparticles at 1, 3, and 6 months. Confocal microscope images display host tissue integration into scaffolds although nonspecificity of GFP does not allow for quantification of integration. However, the confocal images do allow for spatial observation of host tissue migration into the scaffolds at different depths of penetration. The study concludes that the nanoparticle scaffolds are biocompatible and promote integration and that the use of GFP expressing swine can aid in visualizing the scaffold/host interface and host cell/tissue migration.
机译：当使用异源细胞外基质（ECM）衍生的支架进行软组织修复时，当前的体内评估方法可能无法清楚区分宿主胶原蛋白和植入的支架，从而难以评估宿主组织的整合和重塑。这项研究的目的是评估与宿主细胞整合和生物相容性与纳米粒子共轭的新型支架，并评估表达猪的绿色荧光蛋白（GFP）作为评估软组织修复材料的新动物模型。将与纳米粒子结合的人源性移植材料皮下植入表达GFP的猪中，通过组织学评分和共聚焦成像评估其生物相容性和组织整合。组织学评分表明在1、3和6个月时有或没有纳米颗粒的支架的生物相容性和重塑。共聚焦显微镜图像显示宿主组织整合到支架中，尽管GFP的非特异性无法量化整合。然而，共聚焦图像确实允许空间观察宿主组织在不同穿透深度下迁移到支架中。该研究得出的结论是，纳米颗粒支架具有生物相容性并促进整合，并且使用表达GFP的猪可以帮助可视化支架/宿主界面和宿主细胞/组织迁移。

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