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Screening of microorganisms from Antarctic surface water and cytotoxicity metabolites from Antarctic microorganisms

机译：从南极地表水中筛选微生物和南极微生物的细胞毒性代谢产物

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Abstract The Antarctic is a potentially important library of microbial resources and new bioactive substances. In this study, microorganisms were isolated from surface water samples collected from different sites of the Antarctic. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay-based cytotoxicity-tracking method was used to identify Antarctic marine microorganism resources for antitumor lead compounds. The results showed that a total of 129 Antarctic microorganism strains were isolated. Twelve strains showed potent cytotoxic activities, among which a Gram-negative, rod-shaped bacterium, designated as N11-8 was further studied. Phylogenetic analysis based on 16S rRNA gene sequence showed that N11-8 belongs to the genus Bacillus . Fermented active products of N11-8 with molecular weights of 1?￠????30 kDa had higher inhibitory effects on different cancaer cells, such as BEL-7402 human hepatocellular carcinoma cells, U251 human glioma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, and MCF-7 human breast carcinoma cells. However, they displayed lower cytotoxicity against HFL1 human normal fibroblast lung cells. However, they displayed lower cytotoxicity against HFL1 human normal fibroblast lung cells. Microscopic observations showed that the fermented active products have inhibitory activity on BEL-7402 cells similar to that of mitomycin C. Further studies indicated that the fermented active products have high pH and high thermal stability. In conclusion, most strains isolated in this study may be developed as promising sources for the discovery of antitumor bioactive substances. The fermented active products of Antarctic marine Bacillus sp. N11- 8 are expected to be applied in the prevention and treatment of cancer.
机译：摘要南极是潜在的重要微生物资源和新生物活性物质库。在这项研究中，从南极不同地点收集的地表水样品中分离出了微生物。利用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-溴化四唑（MTT）法进行细胞毒性跟踪，以鉴定抗肿瘤先导化合物的南极海洋微生物资源。结果表明，共分离到129株南极微生物。 12株菌株显示出有效的细胞毒性活性，其中进一步研究了革兰氏阴性杆状细菌N11-8。基于16S rRNA基因序列的系统发育分析表明，N11-8属于芽孢杆菌属。 N11-8的发酵活性产物，分子量为1 ??????? 30 kDa，对不同的癌细胞，例如BEL-7402人肝细胞癌细胞，U251人神经胶质瘤细胞，RKO人结肠癌细胞， A549人肺癌细胞和MCF-7人乳腺癌细胞。然而，它们显示出对HFL1人正常成纤维细胞肺细胞较低的细胞毒性。然而，它们显示出对HFL1人正常成纤维细胞肺细胞较低的细胞毒性。显微镜观察表明，发酵的活性产物对BEL-7402细胞具有类似于丝裂霉素C的抑制活性。进一步的研究表明，发酵的活性产物具有高pH值和高热稳定性。总之，本研究中分离出的大多数菌株可能被开发为发现抗肿瘤生物活性物质的有希望的来源。南极海洋芽孢杆菌的发酵活性产物。 N11-8有望用于癌症的预防和治疗。

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