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Study on the extraction and purification of glycoprotein from the yellow seahorse, Hippocampus kuda Bleeker

机译：黄海马中的糖蛋白的提取与纯化研究

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AbstractThe optimum parameters of extraction for glycoprotein from seahorse were examined and determined by Box-Behnken combined with ultrasonic extraction technology. Column chromatography of glycoprotein was used for further purification. The optimal extraction conditions of seahorse glycoprotein were extracting time 4.3 h, salt concentration 0.08 mol/L, extracting temperature 73°C, raw material, and water ratio 1:6. At the optimal conditions, the yield of saccharide reached to 1.123%, and the yield of protein reached to 5.898%. For purifying the crude glycoprotein, the stage renounces of DEAE-52 column chromatography were done, respectively, with 0.05, 0.1, 0.5 mol/L NaHCO3 solution, and further purification was done with Sephadex G-100 column chromatography. Finally, two pieces of seahorse glycoprotein were obtained by the column chromatography, that is, HG-11 and HG-21. The saccharide content was 56.7975% and 39.479%, the protein content was 30.5475% and 51.747%, respectively.
机译：摘要采用Box-Behnken结合超声提取技术，对海马糖蛋白的提取工艺进行了优化。糖蛋白的柱色谱法用于进一步纯化。海马糖蛋白的最佳提取条件为提取时间4.3h，盐浓度0.08mol / L，提取温度73°C，原料，水比1：6。在最佳条件下，糖的收率达到1.123％，蛋白质的收率达到5.898％。为了纯化粗糖蛋白，分别用0.05、0.1、0.5 mol / L NaHCO 3 溶液进行DEAE-52柱色谱的分离，然后用Sephadex G-100进行进一步纯化柱层析。最后，通过柱色谱法获得了两片海马糖蛋白，即HG-11和HG-21。糖含量分别为56.7975％和39.479％，蛋白质含量分别为30.5475％和51.747％。

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