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首页> 外文期刊>Journal of medical bacteriology. >Detection of Enterohemorrhagic Escherichia coli Related Genes in E. coli Strains Belonging to B2 Phylogroup Isolated from Urinary Tract Infections in Combination with Antimicrobial Resistance Phenotypes
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Detection of Enterohemorrhagic Escherichia coli Related Genes in E. coli Strains Belonging to B2 Phylogroup Isolated from Urinary Tract Infections in Combination with Antimicrobial Resistance Phenotypes

机译：结合细菌耐药表型从泌尿道感染中分离到的属于B2菌群的大肠杆菌菌株中大肠埃希菌相关基因的检测

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Background: This study was conducted to detect the prevalence of EHEC virulence genes and antimicrobial resistance profile of Escherichia coli strains belonging to B2 phylogroup implicated in Urinary tract infections in Semnan, Iran.Methods: From 240 urine samples 160 E. coli strains were isolated, biochemically. Then, E. coli isolates were examined by Multiplex-PCR for phylogenetic typing and detection of virulence genes (hly, stx1, stx2, eae) associated with Enterohemorrhagic E. coli. Finally, Antimicrobial resistance of E. coli isolates were characterized using Disk Diffusion method. Results: From 160 E. coli isolates, 75 strains (47%) were assigned to B2 phylogenetic group and prevalence of virulence genes were as follow: hly (21.3%), stx1 (16%), stx2 (10.6%) and eae (6.7%), subsequently. Phenotypic antimicrobial resistance of B2 isolates showed that all isolates were sensitive to Meropenem and Furazolidone and then highest frequency of resistance was observed to Streptomycin, Oxytetracycline, Neomycin, Nalidixic acid and Ampicillin (98.7% to 49.3%). Also low resistance prevalence was observed in case of Ceftizoxime, Lincospectin, Imipenem, Chloramphenicol and flurefenicole (16% to 1.3%).Conclusion: The data suggest a high prevalence of antibiotic resistance in UPEC strains belonging to B2 phylogroup even for the antimicrobials using in pet and farm animals and their potential to cause EHEC specific clinical symptoms which may represent a serious health risk since these strains can be transmitted to GI tract and act as a reservoir for other uropathogenic E. coli and commensal strains.
机译：背景：本研究旨在检测伊朗Semnan感染尿路感染的B2菌群E2大肠杆菌EHEC毒力基因的流行情况和抗菌素耐药性。方法：从240个尿液样本中分离出160株大肠杆菌，生化的。然后，通过多重PCR检查大肠杆菌分离株的系统发育分型，并检测与肠出血性大肠杆菌相关的毒力基因（hly，stx1，stx2，eae）。最后，使用圆盘扩散法对大肠杆菌分离株的抗药性进行了表征。结果：从160株大肠杆菌中，将75株（47％）分为B2系统发育组，毒力基因的流行程度如下：hly（21.3％），stx1（16％），stx2（10.6％）和eae（ 6.7％）。 B2分离株的表型抗药性表明，所有分离株均对美洛培南和呋喃唑酮敏感，然后观察到对链霉素，土霉素，新霉素，萘啶酸和氨苄青霉素的耐药率最高（98.7％至49.3％）。头孢唑肟，林科斯汀，亚胺培南，氯霉素和氟苯尼考的​​耐药率也较低（16％至1.3％）。结论：数据表明，即使对于使用B2 phylogroup的UPEC菌株，其耐药性也很高。宠物和农场动物及其引起EHEC特定临床症状的潜力，这可能代表严重的健康风险，因为这些菌株可以传播到胃肠道，并充当其他泌尿致病性大肠杆菌和共生菌株的贮藏库。

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