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Characterization of Streptococcus thermophilus Host Range Phage Mutants

机译：嗜热链球菌宿主范围噬菌体突变体的表征

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To investigate phage-host interactions in Streptococcus thermophilus, a phage-resistant derivative (SMQ-301R) was obtained by challenging a Tn917 library of phage-sensitive strain S. thermophilus SMQ-301 with virulent phage DT1. Mutants of phages DT1 and MD2 capable of infecting SMQ-301 and SMQ-301R were isolated at a frequency of 10?6. Four host range phage mutants were analyzed further and compared to the two wild-type phages. Altogether, three genes (orf15, orf17, and orf18) contained point mutations leading to amino acid substitutions and were responsible for the expanded host range. These three proteins were also identified in both phages by N-terminal sequencing and/or matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The results suggest that at least three phage structural proteins may be involved in phage-host interactions in S. thermophilus.
机译：为了研究嗜热链球菌中的噬菌体-宿主相互作用，通过用强噬菌体DT1攻击噬菌体敏感性嗜热链球菌SMQ-301的Tn917文库，获得了噬菌体抗性衍生物（SMQ-301R）。能够感染SMQ-301和SMQ-301R的噬菌体DT1和MD2突变体的分离频率为10 6。进一步分析了四个宿主范围的噬菌体突变体，并将其与两个野生型噬菌体进行了比较。总之，三个基因（orf15，orf17和orf18）包含导致氨基酸替换的点突变，并负责扩大宿主范围。还通过N端测序和/或基质辅助激光解吸电离飞行时间质谱在两个噬菌体中鉴定了这三种蛋白质。结果表明，至少三个噬菌体结构蛋白可能参与嗜热链球菌的噬菌体-宿主相互作用。

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