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首页> 外文期刊>Journal of Molecular Biology Research >The Effect of TNF-α on the Expression of MMP9 in Human Mesenchymal Bone Marrow-Derived Stem Cells
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The Effect of TNF-α on the Expression of MMP9 in Human Mesenchymal Bone Marrow-Derived Stem Cells

机译：TNF-α对人间充质骨髓衍生干细胞MMP9表达的影响

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Matrix metalloproteinase 9 (MMP9) as the enzyme of adult stem cells secreted from damage cells. In spite of low level of MMP9 enzyme in the mesenchymal stem cells, many inflammatory cytokines stimulation such as TNF- could increase MMP9 level in cells. Current study evaluated the expression of the MMP9 enzyme under the influence of TNF- in human bone marrow mesenchymal stem cells. The human bone marrow mesenchymal stem cells were classified into control and experimental groups. In the experimental groups, various concentrations of the TNF- (1ng/ml and 10ng/ml) were administrated in different times (10 and 24 hours), whereas the control group was not treated with TNF-. MMP9 gene expression was evaluated by Real-Time PCR. TNF- administration in 1ng/ml and 10ng/ml dosage for 10 hours, induced the expression of MMP9 1468.3 and 1782.8 times more than the control group, respectively. After 24h, in comparison between 1ng/ml and 10ng/ml with control groups, MMP9 expression were 442.64 and 1184.4 times more than control group, respectively. In conclusion, the expression rate of the MMP9 gene in bone marrow mesenchymal stem cells might be effected by dosage and time of exposure to TNF-. Furthermore, the time of exposure might have the prominent role in alteration of MMP9 gene expression induction in the mesenchymal stem cells.
机译：基质金属蛋白酶9（MMP9）作为来自损伤细胞分泌的成体干细胞的酶。尽管间充质干细胞中的MMP9酶水平低，但许多炎症细胞因子刺激如TNF-可以增加细胞中的MMP9水平。目前研究评估了在人骨髓间充质干细胞的影响下MMP9酶的表达。将人骨髓间充质干细胞分类为对照和实验组。在实验组中，各种浓度的TNF-（1ng / ml和10ng / ml）在不同的时间（10和24小时）施用，而对照组未用TNF-处理。通过实时PCR评估MMP9基因表达。在1ng / ml和10ng / ml剂量中施用10小时，诱导比对照组的MMP9 1468.3和1782.8倍的表达。在24h之后，与对照组的1ng / ml和10ng / ml相比，MMP9表达分别比对照组分别为442.64和1184.4倍。总之，骨髓间充质干细胞中MMP9基因的表达率可以通过暴露于TNF-的剂量和时间来实现。此外，暴露的时间可能具有在间充质干细胞中MMP9基因表达诱导的改变方面具有突出作用。

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