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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa to Blue Light Irradiation for Possible Role in Antimicrobial Therapy
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Sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa to Blue Light Irradiation for Possible Role in Antimicrobial Therapy

机译：金黄色葡萄球菌的敏感性和假单胞菌铜绿假单胞菌在抗微生物治疗中的可能作用的蓝光辐照

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Due to the escalation of antibiotic resistant pathogens, non-antibiotic approaches to clinical treatment are being investigated, particularly phototherapy. In this study, 470 nm blue light was examined as an antimicrobial agent against various isolates of S. aureus and P. aeruginosa in vitro. Aim: To determine the adequacy of blue light as an antimicrobial agent against the two pathogens infamous for rapid development of drug resistance. Materials and Methods: ATCC strains of each organism, along with 25 strains of each from patient isolates, were collected. Isolates were suspended in peptone water at 0.5McF, and then inoculated on plates of Mueller-Hinton agar (control and experimental plate). Antibiotic sensitivity of each isolate was determined with 7 common antibiotics, following which, experimental plates were irradiated with a 470 nm Light Emitting Diode (LED) for 60 minutes. Plates were then placed in incubators overnight at 37°C. Zones of inhibition for each experimental plate were compared with the control to determine any action of the blue LED with the antibiotics. Results of the study were analysed using paired t-test where p<0.05 and was calculated for all drugs which showed significant increase in zones of inhibition following 470 nm blue light irradiation. Results: Results demonstrated an increase in zones by 0 to 6 mm, though this was predominantly seen in isolates of P.aeruginosa . The action of the LED was particularly significant with Linezolid for S.aureus and Imipenem for P.aeruginosa , where there was a mean increase of 2 mm and 3 mm, respectively. Comparison of Ciprofloxacin in both pathogens demonstrated a greater increase of zones in plates of P.aeruginosa (2.23 cm) as compared to S.aureus (1.27 cm), suggesting greater sensitivity of this organism to the 470 nm light. Conclusion: This study determined that 470 nm blue light does demonstrate a species-specific inhibitory effect on S.aureus and P.aeruginosa and can act synergistically with antibiotics. With further research regarding its mechanism of action and safety, blue light therapy may be implemented into clinical treatment of skin, wound and burn infections as an adjunct to antibiotics.
机译：由于抗生素抗性病原体的升级，正在研究临床治疗的非抗生素方法，特别是光疗法。在该研究中，将470nm的蓝光被检查为抗微生物剂，抵抗各种分离物的抗微生物剂。金黄色葡萄球菌和 p。铜绿假单胞菌体外。 目的：确定蓝光作为抗微生物剂的充分性，针对两种病原体臭名病原体，以快速发展耐药性。 材料和方法：收集每种生物的ATCC菌株以及来自患者分离株的25个菌株。将分离物悬浮在0.5MCF的蛋白胨水中，然后接种在穆勒 - 六烯琼脂（对照和实验板）的板上。用7种常见的抗生素测定每种分离物的抗生素敏感性，然后用470nm发光二极管（LED）照射实验板60分钟。然后将板置于37℃的培养箱中过夜。将每个实验板的抑制区与对照进行比较，以确定蓝色LED与抗生素的任何作用。通过配对的T检验分析研究结果，其中P <0.05并针对所有药物计算，显示出470 nm蓝光照射后的抑制区显着增加。 结果：结果表明，区域增加0至6mm，但这主要是在 P.aeruginosa的分离物中看到的。 LED的作用与LINEZOLID为 S.AUREUS和IMIPENEM的LINZOLID，分别为2mm和3mm的平均增加。与 S.aureus（1.27cm）相比，Ciphofloxacin在两种病原体中的比较表明，相比， p.aeruginosa（2.23cm）的平板中的区域增加了更大的增加。表明该生物体对470nm光的更大敏感性。 结论：该研究确定了470nm的蓝光确实证明了对β.aureus和 p.aeruginosa的特异性抑制作用，并且可以用抗生素协同作用。通过进一步研究其作用和安全机制，可以将蓝光疗法实施到皮肤，伤口和烧伤感染的临床治疗中，作为抗生素的辅助。

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