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首页> 外文期刊>Food microbiology >Sensitive enumeration of Listeria monocytogenes and other Listeria species in various naturally contaminated matrices using a membrane filtration method
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Sensitive enumeration of Listeria monocytogenes and other Listeria species in various naturally contaminated matrices using a membrane filtration method

机译：使用膜过滤法敏感计数各种自然污染基质中的单核细胞增生李斯特菌和其他李斯特菌

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摘要

For the enumeration of Listeria monocytogenes (L. monocytogenes) in food, a sensitive enumeration method has been recently developed. This method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate L. monocytogenes. An evaluation of this method was performed with several categories of foods naturally contaminated with L. monocytogenes. The results obtained with this technique were compared with those obtained from the modified reference EN ISO 11290-2 method for the enumeration of L. monocytogenes in food, and are found to provide more precise results. In most cases, the filtration method enabled to examine a greater quantity of food thus greatly improving the sensitivity of the enumeration. However, it was hardly applicable to some food categories because of filtration problems and background microbiota interference.
机译：为了对食品中的单核细胞增生李斯特菌（L. monocytogenes）进行计数，最近开发了一种灵敏的计数方法。该方法基于食品悬浮液的膜过滤，然后将过滤器转移到选择性培养基上以计数单核细胞增生李斯特菌。该方法的评估是对几种自然污染了单核细胞增生李斯特菌的食品进行的。将该技术获得的结果与从改良的参考EN ISO 11290-2方法中获得的食品中单核细胞增生李斯特氏菌计数的结果进行了比较，发现它们提供了更精确的结果。在大多数情况下，过滤方法可以检查更多的食物，从而大大提高了枚举的敏感性。然而，由于过滤问题和背景微生物群干扰，它几乎不适用于某些食品。

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  • 来源
    《Food microbiology》 |2015年第6期|171-177|共7页
  • 作者

    Lena Barre; Emilie Brasseur; Camille Doux; Bertrand Lombard; Nathalie Gnanou Besse;

  • 作者单位

    French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du General de Gaulle, 94706 Maisons Alfort cedex, France;

    French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du General de Gaulle, 94706 Maisons Alfort cedex, France;

    French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du General de Gaulle, 94706 Maisons Alfort cedex, France;

    French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du General de Gaulle, 94706 Maisons Alfort cedex, France;

    French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du General de Gaulle, 94706 Maisons Alfort cedex, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Listeria monocytogenes; Enumeration; Filtration; Microbiological methods; Food;

    机译：李斯特菌;列举;过滤;微生物学方法;餐饮;

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