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首页> 外文期刊>Food microbiology >Polyphasic Study Of Microbial Communities Of Two Spanish Farmhouse Goat's Milk Cheeses From Sierra De Aracena
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Polyphasic Study Of Microbial Communities Of Two Spanish Farmhouse Goat's Milk Cheeses From Sierra De Aracena

机译：塞拉利昂德阿拉塞纳市的两个西班牙农家山羊的牛奶干酪的微生物群落多相研究

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The microbial communities present in 2 different types- of farmhouse goats' milk cheese from the Aracena mountains (southwest Spain), Quesailla Arochena (hard cheese) and Torta Arochena (soft cheese), have been studied using both culture-dependent and culture-independent techniques. All bacterial isolates were clustered by using randomly amplified polymorphic DNA (RAPD) and identified by 16S rRNA gene sequencing, species-specific PCR and multiplex PCR. Thus a total of 26 different species were identified, the majority belonging to the lactic-acid bacteria (LAB), mainly represented by Lactococcus lactis and Lactobacillus species such as Lactobacillus plantarum and Lactobacillus paracasei, together with a significant proportion of enterococci. Amongst the non-lactic-acid bacteria (NLAB), which represented 37% of the isolates in Torta Arochena, enterobacteria were the most important, Hafnia alvei and Serratia liquefaciens being the predominant species in. Quesailla Arochena and Torta Arochena respectively. Moreover, RAPD analysis of the isolates revealed that most of the genotypes were specific to one of the cheeses, although a few genotypes common to both cheeses were found.rnThe culture-independent study carried out by temporal-temperature-gradient gel electrophoresis (TTGE) with 2 target genes, rRNA 16S and rpoB, revealed less species diversity but L. lactis and Lb. plantarum were also predominant. Nevertheless, TTGE carried out using RNAr 16S also detected some organisms that had not been isolated by the culture-dependent method, such as Leuconostoc lactis and Mycoplasma agalactie in Quesailla Arochena. Although TTGE of the rpoB gene revealed less species diversity, it did lead to the detection of previously non-isolated species, such as Ln. lactis in Quesailla Arochena. Apart from this, the fingerprinting of Lactobacillus populations by length-heterogeneity PCR showed the predominance of the Lb. plantarum group, followed by Lactobacillus curvatus and, in smaller quantities, Lb. paracasei in Torta Arochena. From our results we may conclude that both types of methods complement each other and offer a more complete vision of the microbial diversity of these ecosystems.
机译：研究了两种文化类型的微生物群落-来自阿拉塞纳山脉（西班牙西南部），Quesailla Arochena（硬奶酪）和Torta Arochena（软奶酪）的两种农舍山羊奶奶酪，均使用了与文化相关和与文化无关的方法技术。所有细菌分离株均使用随机扩增的多态性DNA（RAPD）进行聚类，并通过16S rRNA基因测序，物种特异性PCR和多重PCR进行鉴定。因此，总共鉴定出26种不同的菌种，其中大部分属于乳酸菌（LAB），主要由乳酸乳球菌和植物乳杆菌和副干酪乳杆菌等乳杆菌种代表，并且肠球菌比例也很高。在非乳酸菌（NLAB）中，其占Torta Arochena菌株的37％，其中肠杆菌是最重要的，Hafnia alvei和Serratia liquefaciens是Quesailla Arochena和Torta Arochena中的主要菌种。此外，对分离株的RAPD分析表明，尽管发现了两种干酪均具有的几种基因型，但大多数基因型都对一种干酪具有特异性.rnn通过时间-温度梯度凝胶电泳（TTGE）进行的与培养无关的研究具有2个靶基因的rRNA 16S和rpoB揭示了较少的物种多样性，但乳酸乳球菌和Lb。车前草也是主要的。尽管如此，使用RNA干扰素16S进行的TTGE也检测到了一些尚未通过培养依赖性方法分离的生物，如Quesailla Arochena中的乳酸乳球菌和无乳支原体。尽管rpoB基因的TTGE显示较少的物种多样性，但确实导致了对以前非分离物种（如Ln）的检测。 Quesailla Arochena中的乳酸菌。除此之外，通过长度异质性PCR对乳酸杆菌种群的指纹显示出Lb的优势。植物群，其次是弯曲乳杆菌，少量为Lb。 Torta Arochena中的paracasei。根据我们的结果，我们可以得出结论，两种类型的方法都可以互补，并且可以更全面地了解这些生态系统的微生物多样性。

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