• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of the Institute of Brewing >Analysis of bacterial communities in pit mud from Zhijiang Baijiu distillery using denaturing gradient gel electrophoresis and high-throughput sequencing
【24h】

Analysis of bacterial communities in pit mud from Zhijiang Baijiu distillery using denaturing gradient gel electrophoresis and high-throughput sequencing

机译：变性梯度凝胶电泳和高通量测序法分析枝江白酒酒厂窖泥中的细菌群落

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Zhijiang Baijiu is a Chinese strong flavour liquor produced using a traditional Chinese solid state fermentation involving microorganisms in pit mud on cellar walls. In this study, pit mud samples were collected from three different cellars in a Zhijiang Baijiu distillery ranging in age from 10 to 20 and 30 years. The bacterial communities were analysed using denaturing gradient gel electrophoresis, high-throughput sequencing and quantitative real time PCR. These analyses showed that the diversity of the bacterial community in pit mud samples was relatively stable in cellars aged from 20 to 30 years old. High throughput sequencing also revealed that the relative abundance of seven core bacterial families and nearly half of the core bacterial genera were stable in 20 year-old cellars. In cellars of 10 to 30 years, the relative abundance in pit mud of Ruminococcaceae and Clostridium cluster IV increased, while that of Petrimonas and an unclassified Firmicutes decreased. Real time PCR showed that aged pit mud contained more bacteria and Clostridium kiuyveri than young pit mud. It is concluded that the bacterial communities in aged pit mud are more suitable for producing high quality Baijiu. (C) 2019 The Institute of Brewing & Distilling
机译：枝江白酒是一种中国风味浓烈的酒，它是利用中国传统的固态发酵工艺生产的，这种发酵涉及酒窖壁窖泥中的微生物。在这项研究中，从枝江白酒酿酒厂的三个不同的酒窖中收集了窖泥样品，其年龄范围为10至20和30岁。使用变性梯度凝胶电泳，高通量测序和定量实时PCR分析细菌群落。这些分析表明，在20至30岁的酒窖中，矿坑泥浆样品中细菌群落的多样性相对稳定。高通量测序还显示，在20岁的酒窖中，七个核心细菌家族和近一半的核心细菌属的相对丰度稳定。在10至30年的酒窖中，Ruminococcaceae和Clostridium cluster IV的窖泥中的相对丰度增加，而Petrimonas和未分类的Firmicutes的窖泥相对丰度降低。实时PCR显示，老化的泥浆比年轻的泥浆含有更多的细菌和梭状芽胞杆菌。结论是，老窖泥中的细菌群落更适合于生产优质白酒。 （C）2019酿造与蒸馏研究所

著录项

  • 来源
    《Journal of the Institute of Brewing》 |2020年第1期|90-97|共8页
  • 作者

    Tan Guangxun; Hu Yuanliang; Huang Yinna; Liu Huanming; Dong Weiwei; Li Jing; Liu Jianfeng; Peng Nan; Liang Yunxiang; Zhao Shumiao;

  • 作者单位

    Huazhong Agr Univ Coll Life Sci & Technol State Key Lab Agr Microbiol Wuhan 430070 Peoples R China|Zhijiang Baijiu Ind Co Ltd Zhijiang 443200 Peoples R China;

    Huazhong Agr Univ Coll Life Sci & Technol State Key Lab Agr Microbiol Wuhan 430070 Peoples R China|Hubei Normal Univ Coll Life Sci Hubei Key Lab Edible Wild Plants Conservat & Util Huangshi 435002 Hubei Peoples R China;

    Huazhong Agr Univ Coll Life Sci & Technol State Key Lab Agr Microbiol Wuhan 430070 Peoples R China;

    Guangdong Ocean Univ Coll Food Sci & Technol Zhanjiang 524088 Peoples R China;

    Zhijiang Baijiu Ind Co Ltd Zhijiang 443200 Peoples R China;

    Hubei Light Ind Technol Inst Wuhan 430070 Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Pit mud; microbial community; high-throughput sequencing; DGGE; qPCR;

    机译：坑泥;微生物群落;高通量测序DGGE;定量PCR;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号