...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Vacuum Science & Technology >Microfluidics-assisted photo nanoimprint lithography for the formation of cellular bioimprints
【24h】

Microfluidics-assisted photo nanoimprint lithography for the formation of cellular bioimprints

机译：微流体辅助的光纳米压印光刻技术，用于细胞生物压印的形成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Morphology and surface features provide important markers for cell development and reaction to external stimuli. Bioimprint offers a technique for the permanent capture of these features into an UV-curing, biocompatible, methacrylate biopolymer. This material shows excellent replication fidelity and fast setting times. However, the minimum reproducible feature size depends on the skillful application of the liquid prepolymer. To enable the repeatable formation of high-resolution, structurally bioactive cellular bioimprints, a modified process based on the use of microfluidics for integrated cell culture and polymer delivery has been developed. In this article, the authors introduce the process and demonstrate its use for the culture and imaging of Ishikawa endometrial cancer cells. Transfer of a 100 nm thick Cr test pattern with micrometer-scale features into the biopolymer is demonstrated. Replication and atomic force microscopy imaging of imprinted cellular surface features with sub-50 nm resolution is shown. The process has applications in disease diagnostics, in drug discovery, and for the creation of biomimetic cell culture scaffolds.
机译：形态和表面特征为细胞发育和对外部刺激的反应提供了重要的标志。 Bioimprint提供了一种将这些特征永久捕获到紫外线固化，生物相容性甲基丙烯酸酯生物聚合物中的技术。这种材料显示出极好的复制保真度和快速的固化时间。但是，最小可复制特征尺寸取决于液体预聚物的熟练应用。为使高分辨率，结构上具有生物活性的细胞生物印迹能够重复形成，已经开发了一种基于微流控技术进行整合细胞培养和聚合物输送的改良工艺。在本文中，作者介绍了该过程，并演示了其在石川子宫内膜癌细胞的培养和成像中的用途。演示了将具有微米级特征的100 nm厚Cr测试图案转移到生物聚合物中。显示了低于50 nm分辨率的印迹细胞表面特征的复制和原子力显微镜成像。该方法可用于疾病诊断，药物发现以及仿生细胞培养支架的创建。

著录项

  • 来源
    《Journal of Vacuum Science & Technology》 |2010年第6期|p.C6K17-C6K22|共6页
  • 作者

    V. Nock; L. Murray; F. Samsuri; M. M. Alkaisi; J. J. Evans;

  • 作者单位

    Department for Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New Zealand;

    Department for Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New Zealand;

    Department for Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New Zealand;

    Department for Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New Zealand;

    Christchurch School of Medicine and Health Sciences, University of Otago, Christchurch 8140, New Zealand;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号