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首页> 外文期刊>The Kasetsart Journal >Factors Affecting Growth and β-carotene Content of Chlorosarcinopsis sp. (PSU/CHL20) in Batch Culture
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Factors Affecting Growth and β-carotene Content of Chlorosarcinopsis sp. (PSU/CHL20) in Batch Culture

机译：影响绿藻藻生长和β-胡萝卜素含量的因素（PSU / CHL20）批量培养

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The effect of light intensity, potassium nitrate concentrations and temperature on the growth and β-carotene content of Chlorosarcinopsis sp. (PSU/CHL20) were determined. In the first experiment, algal cells were grown on a shaker in Erlenmeyer flasks containing 150 ml of NSIII medium and at a shaking speed of 150 rpm. Potassium nitrate concentrations were 0, 0.1 and 0.2 g l~(-1). Light intensities used were 60, 120 and 180 μmol m~(-2) s~(-1). The light / dark cycle was 16:8 h and the temperature was controlled at 25℃. The optimum light intensity and potassium nitrate concentration for algal growth and β-carotene content was 120 μmol m~(-2) s~(-1) and 0.1 g l~(-1) which produced a maximum cell number of 138 x 10~5 cell ml~(-1) and a β-carotene content of 0.133 mg l~(-1), (0.012 pg cell~(-1)) respectively. In the second experiment, the effect of temperature on the growth and β-carotene content was investigated. In this experiment the light intensity was 120 mmol m~(-2) s~(-1) and the potassium nitrate concentration was 0.1g l~(-1), at temperatures of 25℃, 30℃ and 35℃. Although statistically insignificant (P > 0.05), the algal growth increased slightly with increasing temperature. The highest cell numbers of 148 x 10~5 cell ml~(-1) were obtained at 30℃ on day 14 of cultivation. The maximum β-carotene content of 0.543 mg l~(-1) (0.052 pg cell~(-1)) was achieved at 30℃ and this was significantly higher than that of the culture grown at 25℃ (P < 0.05).
机译：光强度，硝酸钾浓度和温度对绿藻藻生长和β-胡萝卜素含量的影响。 （PSU / CHL20）已确定。在第一个实验中，藻类细胞在装有150 ml NSIII培养基的锥形瓶中的振荡器上以150 rpm的振荡速度生长。硝酸钾的浓度为0、0.1和0.2 g l〜（-1）。使用的光强度为60、120和180μmolm〜（-2）s〜（-1）。亮/暗周期为16：8 h，温度控制在25℃。藻类生长和β-胡萝卜素含量的最佳光强度和硝酸钾浓度为120μmolm〜（-2）s〜（-1）和0.1 gl〜（-1），产生的最大细胞数为138 x 10〜 5细胞ml〜（-1）和β-胡萝卜素含量分别为0.133 mg l〜（-1），（0.012 pg cell〜（-1））。在第二个实验中，研究了温度对生长和β-胡萝卜素含量的影响。在25℃，30℃和35℃的温度下，光强度为120 mmol m〜（-2）s〜（-1），硝酸钾浓度为0.1g l〜（-1）。尽管在统计学上不显着（P> 0.05），但随着温度的升高，藻类的生长略有增加。培养第14天，在30℃下获得最高细胞数为148×10〜5细胞ml〜（-1）。 β-胡萝卜素最大含量在30℃时达到0.543 mg l〜（-1）（0.052 pg cell〜（-1）），明显高于25℃时培养的β-胡萝卜素含量（P <0.05）。

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