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首页> 外文期刊>Plant and Soil >EXOPOLYSACCHARIDE-DEFICIENT MUTANTS OF ASTRAGALI RHIZOBIA ARE SYMBIOTICALLY EFFECTIVE ON ASTRAGALUS SINICUS, AN INDETERMINATE NODULATING HOST
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EXOPOLYSACCHARIDE-DEFICIENT MUTANTS OF ASTRAGALI RHIZOBIA ARE SYMBIOTICALLY EFFECTIVE ON ASTRAGALUS SINICUS, AN INDETERMINATE NODULATING HOST

机译：黄芪根瘤菌的缺乏外多糖的符号在黄芪（一种不确定的结瘤寄主）上象征性地有效。

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摘要

Five exopolysaccharide-deficient mutants were isolated after rhizobial strain 107 was subjected to transposon Tn5 mutagenesis. The amount of EPS produced by the mutants was dramatically decreased to between 3% and 6% of wild-type level. All mutants carried a singel copy of Tn5. Two mutants (NA3 and NA10) were complemented by the R. meliloti exoA gene and the functionally equivalent exoD gene of Rhizobium sp, strain NGR234. Two other mutants (NA7 and NA8) were complemented by the R. meliloti exoB gene and the functionally equivalent NGR234 exoC gene. The remaining mutant (NA11) was not complemented by any exo genes of R. meliloti or Rhizobium NGR234. All mutants induced normal nitrogen-fixing nodules on Astragalus sinicus, an indeterminate nodulating host.
机译：根瘤菌菌株107转座子Tn5诱变后，分离出五个缺乏胞外多糖的突变体。突变体产生的EPS量大大降低至野生型水平的3％至6％。所有突变体均携带Tn5的单拷贝。两个突变体（NA3和NA10）由苜蓿根瘤菌exoA基因和根瘤菌属菌株NGR234的功能等效exoD基因互补。 meliloti exoB基因和功能等效的NGR234 exoC基因补充了另外两个突变体（NA7和NA8）。其余的突变体（NA11）没有任何苜蓿根瘤菌或根瘤菌NGR234的外显子基因互补。所有突变体在不确定的结节宿主黄芪中诱导正常的固氮结节。

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