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首页> 外文期刊>Chemical Senses >Genome and EST Analyses and Expression of a Gene Family with Putative Functions in Insect Chemoreception
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Genome and EST Analyses and Expression of a Gene Family with Putative Functions in Insect Chemoreception

机译：在昆虫化学感受中具有推定功能的基因家族的基因组和EST分析和表达

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Odorant-binding proteins (OBPs)are thought to be responsible for the transport of semiochemicals across hydrophobic interfaces to olfactory receptors.In insects,a second class of OBPs with four conserved cysteines has been variously named as sensory appendage proteins,olfactory segment-D proteins,and chemosensory proteins (CSPs).The physiological functions of these proteins have remained elusive.Here we report a comprehensive survey of both genome and expressed sequence tags (EST)databases.This showed that CSPs are apparently only present in the phylum,Arthropoda,and in two subphyla,Crustacea and Uniramia.This is the first report of a putative CSP in Crustacea and suggests that the origin of these genes predates the divergence of Uniramia and Crustacea.For the Uniramia,we identified 74 new genes encoding putative CSPs of insect species from 10 different orders.Using tissue-specific EST libraries,we have examined the relative expression of putative CSP genes in many tissues from 22 insect species suggesting that the genes are expressed widely.One Drosophila CSPs is expressed sixfold higher in head than other CSPs.One Bombyxmori CSPs was found at a very High level in pheromone gland,and for the first time,six CSPs were identified in B.mori compound eyes.The different frequencies of CSP transcripts were observed between solitary and gregarious EST libraries of Locusta migratoria.
机译：气味结合蛋白（OBPs）被认为是通过疏水性界面将符号化学物质转运到嗅觉受体的原因。在昆虫中，带有四个保守半胱氨酸的第二类OBP被不同地命名为感觉附属蛋白，嗅觉节段D蛋白以及化学感应蛋白（CSP）。这些蛋白的生理功能仍然难以捉摸。在这里，我们报告了对基因组和表达序列标签（EST）数据库的全面调查。这表明CSP显然仅存在于门，节肢动物，这是甲壳纲中一个推定的CSP的首次报道，表明这些基因的起源早于Uniramia和甲壳纲的差异。对于Uniramia，我们鉴定了74个编码昆虫推定的CSP的新基因。来自10个不同阶的物种。使用组织特异性EST文库，我们检查了22例inse的许多组织中推定CSP基因的相对表达ct物种表明该基因被广泛表达。果蝇CSP的头部表达比其他CSP高六倍。在信息素腺中发现一个Bombyxmori CSP的含量很高，并且首次在B中鉴定出六个CSP。家蝇的单眼和群居EST文库之间观察到不同的CSP转录本频率。

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