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Gallus expression in situ hybridization analysis: a chicken embryo gene expression database

机译:鸡胚表达原位杂交分析:鸡胚基因表达数据库

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摘要

With sequencing of the chicken genome largely completed, significant effort is focusing on gene annotation, including acquiring information about the patterns of gene expression. The chicken embryo is ideally suited to provide detailed temporal and spatial expression information through in situ hybridization gene expression analysis in vivo. We have developed the Gallus expression in situ hybridization analysis (GEISHA) database and user interface (http://geisha.arizona.edu) to serve as a centralized repository of in situ hybridization photos and metadata from chicken embryos. This report describes the design and implementation the GEISHA database and Web site and illustrates its usefulness for researchers in the biomedical and poultry science communities. Results from a recent comprehensive expression analysis of microRNA expression in chicken embryos are also presented.
机译:鸡基因组测序已基本完成,大量工作集中在基因注释上,包括获取有关基因表达模式的信息。鸡胚非常适合通过体内原位杂交基因表达分析提供详细的时间和空间表达信息。我们已经开发了Gallus表达原位杂交分析(GEISHA)数据库和用户界面(http://geisha.arizona.edu),可以用作来自鸡胚胎的原位杂交照片和元数据的集中存储库。该报告描述了GEISHA数据库和网站的设计和实现,并说明了其对生物医学和家禽科学界的研究人员的有用性。还提供了最近在鸡胚中对microRNA表达进行全面表达分析的结果。

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