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首页> 外文期刊>Biomaterials Science >Controllable cell adhesion, growth and orientation on layered silk protein films
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Controllable cell adhesion, growth and orientation on layered silk protein films

机译:分层丝蛋白膜上可控的细胞粘附,生长和方向

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摘要

Due to their mechanical stability, biocompatibility and biodegradability, silks are promising materials for various biomedical applications including tissue engineering. Since the shape and the organisation of cells in and on scaffolds both affect their function, we tested patterned silk scaffolds made of three different silk proteins concerning their influence on cell adhesion, growth and orientation. Two different cell lines, BALB/3T3 fibroblasts and C2C12 myoblasts, showed controllable cell adhesion as well as orientation dependent on the silk proteins used and patterns made. Surprisingly, the presence of the integrin binding motif RGD did not influence cell adhesion and orientation on structured silk films, although it did so significantly on flat films.
机译:由于其机械稳定性,生物相容性和生物降解性,丝绸是包括组织工程在内的各种生物医学应用的有前途的材料。由于支架中和支架上细胞的形状和组织均会影响其功能,因此我们测试了由三种不同的丝蛋白制成的图案化丝支架对它们对细胞粘附,生长和方向的影响。两种不同的细胞系BALB / 3T3成纤维细胞和C2C12成肌细胞显示出可控制的细胞粘附性和方向,具体取决于所用的丝蛋白和制备的模式。令人惊讶地,整联蛋白结合基序RGD的存在不影响结构化丝膜上的细胞粘附和取向,尽管它在平面膜上显着。

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