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Concentrations of endogenous prostaglandin F2alpha in boar semen and effect of a 72-h incubation period on exogenous prostaglandin F2alpha concentration in extended boar semen.

机译:公猪精液中内源性前列腺素F2alpha的浓度以及72小时潜伏期对延长公猪精液中外源性前列腺素F2alpha浓度的影响。

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摘要

PGF2alpha in semen has been shown to induce uterine contractions, thereby, facilitating sperm transport during fertilization. Previously, we demonstrated that extended boar semen used in artificial insemination does not increase myometrial contractility, but PGF2alpha supplementation did. In this study, we determined the concentrations of endogenous PGF2alpha in pre-sperm and sperm-rich fractions of the boar ejaculate and examined whether changes in the concentration of exogenous PGF2alpha occurred when added to extended boar semen after 72-h incubation at a 17 degrees C storage temperature. Concentrations of endogenous PGF2alpha (n = 10 boars) in pre-sperm and sperm-rich fractions were 69.6 +/- 7.6 and 58.9 +/- 4.4 pg/ml, respectively. No differences were observed in the concentrations of exogenous PGF2alpha in the extended boar semen at 0 h (59.3 +/- 3.3 microg/ml) and after a 72-h incubation period (52.0 +/- 2.1 microg/ml). These results suggest that the concentration of endogenous PGF2alpha in boarsemen used for artificial insemination is < 100 pg/ml. The concentration of exogenous PGF2alpha in the extended boar semen did not differ after 72 h, which indicates that it is not metabolized during this period of time.
机译:精液中的PGF2α已显示可引起子宫收缩,从而促进受精过程中的精子转运。以前,我们证明了在人工授精中使用的延长公猪精液不会增加子宫肌层的可收缩性,但是PGF2alpha的补充却可以。在这项研究中,我们确定了公猪射精前和精子富集部分中内源性PGF2alpha的浓度,并检查了在17度温育72小时后添加到扩展的公猪精液中是否存在外源性PGF2alpha的浓度变化。 C储存温度。精子前和精子富集部分中内源性PGF2alpha(n = 10头公猪)的浓度分别为69.6 +/- 7.6和58.9 +/- 4.4 pg / ml。在0 h(59.3 +/- 3.3 microg / ml)和72 h潜伏期(52.0 +/- 2.1 microg / ml)后,延伸的公猪精液中外源性PGF2alpha的浓度未见差异。这些结果表明,用于人工授精的公猪中内源性PGF2α的浓度小于100 pg / ml。延长的公猪精液中外源性PGF2α的浓度在72小时后没有变化,这表明它在这段时间内没有被代谢。

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