• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Urologic oncology >p53 immunoreactivity correlates with Ki-67 and bcl-2 expression in renal cell carcinoma.
【24h】

p53 immunoreactivity correlates with Ki-67 and bcl-2 expression in renal cell carcinoma.

机译：p53免疫反应性与肾细胞癌中Ki-67和bcl-2表达相关。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study assessed the relation of proliferation, inhibition of apoptosis, and the p53 tumor suppressor protein expression in clear renal cell carcinoma (RCC). Archival pathological specimens from 43 patients treated for RCC were obtained. Median follow-up for the patients was 52 months (range 2.5 months to 178 months). Immunostaining of paraffin tissue sections was carried out for four different markers: a) Ki-67, a marker for cellular proliferation; b) p53/DO7, c) p53/pAb240, antibodies for the p53 protein; and d) bcl-2, a marker for inhibition of apoptosis (programmed cell death). One thousand cells were counted per slide at 400x magnification. Staining of >/=10% of cells was considered positive and <10% negative. Fisher exact contingency tables were used for correlation between markers, tumor grade and stage. A significant correlation was found between Ki-67 and p53 immunoreactivity samples, P=0.0001. Interestingly, a significant association was found if Ki-67 and bcl-2 scores were combined and correlated with p53, P=0.009. Results showed no correlation between any of the immunohistochemical markers and grade or stage. In addition, Kaplan-Meier survival curves demonstrated no significant difference between patients' tumors that was scored immunoreactive negative vs. positive for Ki-67, p53, or bcl-2. This study indicates that p53 expression correlates with proliferation, and inhibition of programmed cell death in RCC.
机译：这项研究评估了透明肾细胞癌（RCC）中增殖，凋亡抑制和p53肿瘤抑制蛋白表达之间的关系。从43例接受RCC治疗的患者中获取了病理样本。患者的中位随访时间为52个月（2.5个月至178个月）。对四种不同的标记物进行石蜡组织切片的免疫染色：a）Ki-67，一种细胞增殖的标记物; b）p53 / DO7，c）p53 / pAb240，p53蛋白的抗体; d）bcl-2，一种抑制细胞凋亡（程序性细胞死亡）的标志物。每个玻片以400x放大倍数计数一千个细胞。 ＞ / ＝ 10％的细胞染色被认为是阳性而＜10％的阴性。 Fisher精确列联表用于标记，肿瘤等级和阶段之间的相关性。发现Ki-67和p53免疫反应性样品之间存在显着相关性，P = 0.0001。有趣的是，如果将Ki-67和bcl-2得分结合并与p53相关，则发现显着相关性，P = 0.009。结果显示，任何免疫组织化学标记物与等级或阶段之间均无相关性。此外，Kaplan-Meier生存曲线表明，对于Ki-67，p53或bcl-2，免疫反应阴性与阳性的患者肿瘤之间无显着差异。这项研究表明p53表达与RCC的增殖和抑制程序性细胞死亡有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号