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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Study on interactions of endocrine disruptors with estrogen receptor using fluorescence polarization
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Study on interactions of endocrine disruptors with estrogen receptor using fluorescence polarization

机译：利用荧光偏振研究内分泌干扰物与雌激素受体的相互作用

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摘要

In this study, we examined the affinities of many (21) compounds such as hormones, pharmaceuticals, industrial chemicals, and phytoestrogens to the estrogen receptor (ER) by ER binding assay using fluorescence polarization (FP). This method is based on the competitive binding assay using fluorescein-labeled estradiol (F-E2), in which the polarization values decreased with the addition of the compounds (competitors). The obtained sigmoidal inhibition curves were transformed into the pseudo-Hill plots, and the concentrations at 50% inhibition (IC50) and Hill coefficients were obtained from the regression equations. We examined the relationship between the chemical structures and estrogenic activities, and finally classified the tested compounds into three categories, agonists, partial agonists and antagonists based on their Hill coefficients. [References: 31]
机译：在这项研究中，我们通过使用荧光偏振（FP）的ER结合测定，检查了许多（21）化合物，例如激素，药物，工业化学品和植物雌激素对雌激素受体（ER）的亲和力。此方法基于使用荧光素标记的雌二醇（F-E2）的竞争结合测定，其中极化值随添加化合物（竞争者）而降低。将获得的S形抑制曲线转换成伪希尔曲线，并从回归方程中获得50％抑制浓度（IC50）和希尔系数。我们检查了化学结构与雌激素活性之间的关系，最后根据其Hill系数将被测化合物分为三类：激动剂，部分激动剂和拮抗剂。 [参考：31]

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