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首页> 外文期刊>The European Journal of Neuroscience >Neurons expressing relaxin 3/INSL 7 in the nucleus incertus respond to stress.
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Neurons expressing relaxin 3/INSL 7 in the nucleus incertus respond to stress.

机译：在无核中表达松弛素3 / INSL 7的神经元对压力作出反应。

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摘要

Relaxin 3/INSL 7 has recently been identified as a new member of the insulin/relaxin superfamily. Although it was reported to be dominantly expressed in the brain, its detailed distribution and function in the central nervous system are still obscure. In the present study we demonstrated that in the rat relaxin 3 was mainly expressed in neurons of the nucleus incertus (NI) of the median dorsal tegmental pons. Other relaxin 3-expressing neurons were scattered in the pontine raphe nucleus, the periaqueductal gray and dorsal area to the substantia nigra in the midbrain reticular formation. Relaxin 3-immunoreactive fibers projected particularly densely in the septum, hippocampus, lateral hypothalamus and intergeniculate leaflet of the thalamus. Ultrastructural examination revealed that relaxin 3 was localized in the dense-cored vesicles in the perikarya and was also observed in the synaptic terminals of axons. As almost all relaxin 3-containing neurons express corticotropin-releasing factor (CRF) type 1 receptor in the NI, we examined the response of relaxin 3 neurons to intracerebroventricular administration of CRF; 65% of relaxin 3 neurons expressed c-Fos 2 h after intracerebroventricular administration of 1 microg CRF. We then confirmed that c-Fos was induced in 60% of relaxin 3 neurons in the NI and the expression of relaxin 3 mRNA increased significantly in the NI after water-restraint stress. Collectively, these results suggest that relaxin 3 produced in the NI is released from nerve endings and is involved in the regulation of the stress response.
机译：最近，松弛素3 / INSL 7被确定为胰岛素/松弛素超家族的新成员。尽管据报道它在大脑中占主导地位，但它在中枢神经系统中的详细分布和功能仍然不清楚。在本研究中，我们证明了在大鼠中，松弛素3主要在正中背侧脑桥的不活动核（NI）神经元中表达。其他表达松弛素3的神经元散布在脑桥网状核，中脑网状结构中黑膜旁的导水管周围灰色和背侧区域。松弛素3免疫反应性纤维在隔膜，海马，下丘脑外侧和丘脑的间质小叶中特别密集地突出。超微结构检查显示松弛素3定位在核仁周围的密集核小泡中，并且在轴突的突触末端也观察到。由于几乎所有含松弛素3的神经元在NI中均表达促肾上腺皮质激素释放因子（CRF）1型受体，因此我们检查了松弛素3神经元对脑室内CRF的反应。脑室内注射1 microg CRF后2小时，有65％的松弛素3神经元表达c-Fos。然后，我们证实了水分抑制胁迫后，NI中60％的松弛素3神经元诱导了c-Fos，并且在NI中松弛素3 mRNA的表达显着增加。总的来说，这些结果表明在NI中产生的松弛素3从神经末梢释放并参与应激反应的调节。

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