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Oil-in-water microemulsion LC determination of pharmaceuticals using gradient elution

机译：梯度洗脱法测定水包油微乳LC

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Efficient and novel oil-in-water microemulsion HPLC (MELC) separations of a range of solutes have been performed on conventional reversed-phase HPLC columns using gradient elution. This work follows previous successful separations using isocratic oil-in-water MELC [1]. It was found that by changing certain variables, peak-peak resolution, separation selectivity, efficiency and solute retention could be manipulated. The method was compatible with very low UV detection wavelengths. A robust separation method was developed for the quantitative analysis of 2 steroids in a combination-inhaled product for asthma. The method offered similar chromatography and run time when compared with conventional HPLC modes, thus demonstrating its potential for routine use. Stability-indicating methods were developed to separate synthetic and degradative impurities from the main component peaks in 4 pharmaceutical products. The methods offered quicker analysis times and equivalent selectivity to conventional HPLC modes. In developing the separations the effect on the chromatography of varying the operating parameters was studied.
机译：在常规的反相HPLC色谱柱上，使用梯度洗脱法对一系列溶质进行了高效新颖的水包油微乳HPLC（MELC）分离。这项工作是继先前使用等度水包油MELC [1]成功分离之后的结果。发现通过改变某些变量，可以控制峰-峰分辨率，分离选择性，效率和溶质保留。该方法与非常低的紫外线检测波长兼容。开发了一种可靠的分离方法，用于定量分析联合吸入的哮喘产品中的两种类固醇。与常规HPLC模式相比，该方法提供了相似的色谱法和运行时间，因此证明了其常规用途的潜力。开发了指示稳定性的方法，以从4种药品中的主要成分峰中分离出合成和降解杂质。该方法提供了更快的分析时间和与常规HPLC模式相当的选择性。在开发分离过程中，研究了不同操作参数对色谱的影响。

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