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首页> 外文期刊>Yeast >The KlPMR1 gene of Kluyveromyces lactis encodes for a P-type Ca2+-ATPase.
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The KlPMR1 gene of Kluyveromyces lactis encodes for a P-type Ca2+-ATPase.

机译：乳酸克鲁维酵母的KlPMR1基因编码一个P型Ca2 + -ATPase。

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摘要

A novel P-type Ca2+-ATPase gene was cloned and sequenced in the yeast Kluyveromyces lactis. The gene was named KlPMR1 and is localized on chromosome I. The putative gene product contains 936 residues and has an estimated molecular weight of 102 437Da. Analysis of the deduced amino acid sequence (KlPmr1p) indicated that the encoded protein retains all the highly conserved domains characterizing the P-type ATPases. KlPmr1p shares 71% amino acid identity with Pmr1p of Saccharomyces cerevisiae, 62% with HpPmr1p of Hansenula polymorpha, 56% with YlPmr1p of Yarrowia lipolytica and 52% with the Ca2+-ATPase encoded for by the SPCA1 gene of Rattus norvegicus; these similarities place KlPmr1p in the SPCA group (secretory pathway Ca2+-ATPase) of the P-typeATPases. The K. lactis strain harbouring the Klpmr1 disrupted gene is not able to grow in the presence of low calcium concentrations and shows hypersensitivity to high concentrations of EGTA in the medium. These defects are relieved by PMR1 of S. cerevisiae on a centromeric plasmid, demonstrating that KlPMR1 encodes for a functional Pmr1p homologue. The sequence described can be retrieved under EMBL Accession No. AJ001018.
机译：一个新的P型Ca2 + -ATPase基因被克隆并在酵母克鲁维酵母中测序。该基因名为KlPMR1，位于染色体I上。推定的基因产物包含936个残基，估计分子量为102 437Da。对推导的氨基酸序列（K1Pmr1p）的分析表明，编码的蛋白质保留了表征P型ATP酶的所有高度保守的结构域。 KlPmr1p与酿酒酵母的Pmr1p具有71％的氨基酸同一性，多形汉逊酵母的HpPmr1p具有62％的氨基酸，解脂耶氏酵母的YlPmr1p具有56％的氨基酸，与褐家鼠的SPCA1基因编码的Ca2 + -ATPase具有52％的氨基酸同一性。这些相似之处将KlPmr1p置于P型ATPase的SPCA组（分泌途径Ca2 + -ATPase）中。带有Klpmr1破坏基因的乳酸克鲁维酵母菌株在低钙浓度下不能生长，并且对培养基中高浓度EGTA表现出超敏性。这些缺陷通过着丝粒质粒上啤酒酵母的PMR1缓解，表明K1PMR1编码功能性Pmr1p同源物。可以在EMBL登录号AJ001018下检索描述的序列。

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