• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Yeast >Regulation of the expression of the Kluyveromyces lactis PDC1 gene: Carbonsource-responsive elements and autoregulation
【24h】

Regulation of the expression of the Kluyveromyces lactis PDC1 gene: Carbonsource-responsive elements and autoregulation

机译：乳酸克鲁维酵母PDC1基因表达的调节：碳源响应元素和自动调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The yeast Kluyveromyces lactis has a single structural gene coding for pyruvate decarboxylase (KlPDC1). In order to study the regulation of the expression of KlPDC1, we have sequenced (EMBL Accession No. Y15435) its promoter and have fused the promoter to the reporter gene lacZ from E. coli. Transcription analysis in a Klpdc1 Delta strain showed that KlPDC1 expression is subject to autoregulation. The PDC1 gene from Saccharomyces cerevisiae was able to complement the Rag(-) phenotype of the Klpdc1 Delta mutant strain and it could also repress transcription of the KlPDC1-lacZ fusion on glucose. A deletion analysis of the promoter region was performed to study carbon source-dependent regulation and revealed that at least two cis-acting regions are necessary for full induction of gene expression on glucose. Other cis-elements mediate repression on ethanol. Copyright (C) 1999 John Wiley & Sons, Ltd.
机译：酵母乳酸克鲁维酵母具有编码丙酮酸脱羧酶（KIPDC1）的单个结构基因。为了研究KlPDC1表达的调控，我们对其启动子进行了测序（EMBL登录号Y15435），并将该启动子与大肠杆菌的报告基因lacZ融合。在Klpdc1 Delta菌株中的转录分析表明，KlPDC1的表达受到自动调节。来自酿酒酵母的PDC1基因能够补充Klpdc1 Delta突变株的Rag（-）表型，并且还可以抑制葡萄糖上的KlPDC1-lacZ融合蛋白的转录。进行启动子区域的缺失分析以研究碳源依赖性调节，并揭示了至少两个顺式作用区域对于完全诱导葡萄糖上的基因表达是必需的。其他顺式元素介导对乙醇的抑制。版权所有（C）1999 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号