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首页> 外文期刊>Vaccine >The effect of vaccination with a recombinant Nippostrongylus brasiliensis acetylcholinesterase on infection outcome in the rat
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The effect of vaccination with a recombinant Nippostrongylus brasiliensis acetylcholinesterase on infection outcome in the rat

机译：重组巴西夜蛾乙酰胆碱酯酶疫苗接种对大鼠感染结局的影响

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摘要

Nippostrongylus brasiliensis, the rodent hookworm, is a commonly used model of gastrointestinal nematode infection. This parasite, hookworms and several livestock nematode parasites of importance secrete distinct forms of acetylcholinesterases (AChE) that have been ascribed a putative parasite protective function. We tested the hypothesis that vaccination with the secreted enzyme would be deleterious to the parasite. Rats were immunised with a recombinant AChE isoform B via the subcutaneous, intra-peritoneal and intra-nasal routes using different adjuvants dependent on the mode of delivery and subsequently challenged with N. brasiliensis. Rats immunised via the subcutaneous and intra-nasal routes showed a modest but significant decrease in egg output of between 23 and 48%. This was mirrored by differences in the titre of specific antibody isotypes in the serum and mucosa following infection and serum from vaccinated animals was demonstrated to inhibit the activity of recombinant and native AChE. The utility of this model for future development of hookworm and veterinary nematode vaccines is discussed.
机译：巴西夜蛾（Nippostrongylus brasiliensis）是啮齿类钩虫，是胃肠道线虫感染的常用模型。这种寄生虫，钩虫和重要的几种家畜线虫寄生虫分泌出不同形式的乙酰胆碱酯酶（AChE），这被认为是假定的寄生虫保护功能。我们检验了用分泌的酶进行疫苗接种会对寄生虫有害的假设。使用取决于递送方式的不同佐剂，通过皮下，腹膜内和鼻内途径用重组AChE同工型B对大鼠进行免疫，然后用巴西猪笼草对其进行攻击。通过皮下和鼻内途径免疫的大鼠的卵产量适度但显着下降了23％至48％。感染后血清和粘膜中特异性抗体同种型效价的差异反映了这一点，并证明了来自接种动物的血清可抑制重组AChE和天然AChE的活性。讨论了该模型在钩虫和兽用线虫疫苗未来开发中的实用性。

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