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首页> 外文期刊>Journal of Experimental Botany >Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice
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Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice

机译：S-结构域受体样激酶胞外结构域的过表达改善水稻穗构型和籽粒产量

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摘要

The S-domain receptor kinase (SRK) comprises a highly polymorphic subfamily of receptor-like kinases (RLKs) originally found to be involved in the self-incompatibility response in Brassica. Although several members have been identified to play roles in developmental control and disease responses, the correlation between SRKs and yield components in rice is still unclear. The utility of transgenic expression of a dominant negative form of SRK, OsLSK1 (Large spike S-domain receptor like Kinase 1), is reported here for the improvement of grain yield components in rice. OsLSK1 was highly expressed in nodes of rice and is a plasma membrane protein. The expression of OsLSK1 responded to the exogenous application of growth hormones, to abiotic stresses, and its extracellular domain could form homodimers or heterodimers with other related SRKs. Over-expression of a truncated version of OsLSK1 (including the extracellular and transmembrane domain of OsLSK1 without the intracellular kinase domain) increased plant height and improve yield components, including primary branches per panicle and grains per primary branch, resulting in about a 55.8% increase of the total grain yield per plot (10 plants). Transcriptional analysis indicated that several key genes involved in the GA biosynthetic and signalling pathway were up-regulated in transgenic plants. However, full-length cDNA over-expression and RNAi of OsLSK1 transgenic plants did not exhibit a detectable visual phenotype and possible reasons for this were discussed. These results indicate that OsLSK1 may act redundantly with its homologues to affect yield traits in rice and manipulation of OsLSK1 by the dominant negative method is a practicable strategy to improve grain yield in rice and other crops.
机译：S结构域受体激酶（SRK）包含受体样激酶（RLKs）的高度多态性亚家族，最初被发现与芸苔属植物的自交不亲和反应有关。尽管已经确定了几个成员在发育控制和疾病反应中发挥作用，但水稻中SRKs与产量构成之间的相关性仍不清楚。据报道，SRK显性负型OsLSK1（大穗S域受体，如激酶1）的转基因表达可用于改善水稻的籽粒产量。 OsLSK1在水稻节中高表达，是一种质膜蛋白。 OsLSK1的表达对生长激素的外源应用，非生物胁迫产生响应，其细胞外结构域可与其他相关SRKs形成同二聚体或异二聚体。截短版本的OsLSK1（包括没有细胞内激酶结构域的OsLSK1的胞外和跨膜结构域）的过表达增加了株高并改善了产量成分，包括每个穗的主要分支和每个主要分支的谷粒，导致产量增加了约55.8％。每个地块的总谷物产量（10株植物）。转录分析表明，在转基因植物中，参与GA生物合成和信号传导途径的几个关键基因被上调。然而，OsLSK1转基因植物的全长cDNA过表达和RNAi没有表现出可检测的视觉表型，因此对此进行了讨论。这些结果表明，OsLSK1可能与其同源物冗余地起作用，从而影响水稻的产量性状，通过显性负性方法操纵OsLSK1是提高水稻和其他农作物谷物产量的可行策略。

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