In this study, medium-chain fatty acid (MCFA) liposomes were prepared by the film ultrasonic dispersion, modified ethanol injection, and reverse-phase evaporate methods. The results indicated that the liposomes prepared by the thin-film ultrasonic dispersion method had a high entrapment efficiency of 82.7% and a good distribution in size diameters. The MCFA liposomes were freeze-dried and the optimal preparation conditions of freeze-drying were as follows: The cryoprotectants were mannitol and sucrose (1:1 w/w), the hydrated medium was distilled water, and the freeze-drying time was 48 hours. Under these conditions, the freeze-dried MCFA liposomes had a perfect appearance, a small particle size, and high encapsulation efficiency. The mean diameters were 251.1 and 265.3 nm, and the encapsulation efficiencies were 80.5 and 79.2% for freshly prepared and reconstituted liposomes, respectively.
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机译：在这项研究中，中膜脂肪酸（MCFA）脂质体是通过薄膜超声分散，改良乙醇注射和反相蒸发法制备的。结果表明，采用薄膜超声分散法制备的脂质体包封率高，达82.7％，粒径分布良好。将MCFA脂质体冷冻干燥，冷冻干燥的最佳制备条件如下：冷冻保护剂为甘露醇和蔗糖（1：1 w / w），水合介质为蒸馏水，冷冻干燥时间为48小时。在这些条件下，冻干的MCFA脂质体具有完美的外观，小的粒径和高的包封效率。平均直径为251.1和265.3 nm，对于新鲜制备和重构的脂质体，包封效率分别为80.5％和79.2％。
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