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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Long-term human coronavirus-myelin cross-reactive T-cell clones derived from multiple sclerosis patients.
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Long-term human coronavirus-myelin cross-reactive T-cell clones derived from multiple sclerosis patients.

机译：源自多发性硬化症患者的长期人冠状病毒-髓磷脂交叉反应性T细胞克隆。

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Autoimmune reactions associated with MS involve genetic and environmental factors. Because murine coronaviruses induce an MS-like disease, the human coronaviruses (HCoV) are attractive candidates as environmental factors involved in a demyelinating pathology. We previously reported the isolation of HCoV-229E/myelin basic protein (MBP) cross-reactive T-cell lines (TCL) in MS patients. To investigate antigenic cross-reactivity at the molecular level, 155 long-term T-cell clones (TCC) were derived from 32 MS patients by in vitro selection with MBP, proteolipid protein (PLP) or HCoV (strains 229E and OC43). Overall, 114 TCC were virus-specific, 31 were specific for myelin Ag and 10 other were HCoV/myelin cross-reactive. Twenty-eight virus-specific TCC and 7 myelin-specific TCC were obtained from six healthy donors. RACE RT-PCR amplification of the Vbeta chains of five of ten the cross-reactive TCC confirmed clonality and sequencing identified the CDR3 region associated with cross-reactivity. Our findings have promising implications in the investigation of the role of molecular mimicry between coronaviruses and myelin in MS as a mechanism related to disease initiation or relapses.
机译：与MS相关的自身免疫反应涉及遗传和环境因素。由于鼠冠状病毒可诱发MS样疾病，因此人类冠状病毒（HCoV）作为脱髓鞘病理学所涉及的环境因素是有吸引力的候选者。我们以前曾报道过MS患者中HCoV-229E /髓鞘碱性蛋白（MBP）交叉反应性T细胞系（TCL）的分离。为了在分子水平上研究抗原交叉反应性，通过体外选择MBP，蛋白脂蛋白（PLP）或HCoV（229E和OC43株）从32位MS患者中获得了155个长期T细胞克隆（TCC）。总体而言，有114种TCC是病毒特异性的，其中31种是针对髓磷脂Ag的特异性，另外10种是HCoV /髓磷脂交叉反应的。从六名健康供体中获得了28种病毒特异性TCC和7种髓磷脂特异性TCC。十个交叉反应的TCC中有五个的Vbeta链的RACE RT-PCR扩增证实了克隆性和测序确定了与交叉反应相关的CDR3区。我们的发现在冠状病毒和髓鞘之间的分子模拟在MS中作为与疾病发生或复发相关的机制中的作用的研究中具有前途。

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