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首页> 外文期刊>Comparative Medicine >Differentiation of Neural Cells in the Fetal Cerebral Cortex of Cynomolgus Monkeys (Macaca fascicularis)
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Differentiation of Neural Cells in the Fetal Cerebral Cortex of Cynomolgus Monkeys (Macaca fascicularis)

机译：食蟹猴胎儿大脑皮层神经细胞的分化

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摘要

Proliferation and programmed cell death are important in the formation of morphologic structures and functional activity during CNS development. We used immunohistochemical and TUNEL methods to examine the proliferation and differentiation of neural cells in, distribution of apoptotic cells in, and microglial cell involvement in the removal of apoptotic cells from the fetal cerebral cortex of cynomolgus monkeys. At embryonic day (E) 50 and E80, the neuroepithelium contained many mitotic cells. Cells staining for PCNA (a nuclear marker of proliferating cells) were prominent in the proliferative zone, whereas cells positive for NeuN (a neuron-specific marker) were absent. GFAP staining for glial cells was positive in the neuroepithelium and radial glial fibers. Iba1-positive cells (that is, macrophages and microglia) were distributed throughout all regions at all time points but accumulated especially in the ventricular zone at E80. Apoptotic morphology (at E80) and TUNEL-positive cells (that is, containing DNA fragmentation; at E50 and E80) were observed also. At E120 and E150, most PCNA-positive cells were in the ventricular zone, and NeuN-positive cells were prominent in all layers except layer I-II at E120. GFAP immunoreactivity was detected mainly in cells with fine processes in the white matter. Neither apoptosis nor TUNEL-positive cells were detected at either E120 or E150. These results suggest that proliferation, migration, and neural cell death occur during midgestation (that is, E50 to E80) in fetal brain of cynomolgus macaques, whereas differentiation and maturation of neural cells occur after midgestation (E80).
机译：增殖和程序性细胞死亡在中枢神经系统发育过程中的形态结构和功能活性形成中很重要。我们使用免疫组化和TUNEL方法检查了猕猴胎儿大脑皮层中神经细胞的增殖和分化，凋亡细胞的分布以及小胶质细胞参与了凋亡细胞的清除。在胚胎第50天和E80天，神经上皮细胞含有许多有丝分裂细胞。 PCNA（增殖细胞的核标志物）的细胞染色在增殖区中突出，而NeuN（神经元特异性标志物）阳性的细胞不存在。神经胶质上皮细胞和radial神经胶质纤维的GFAP染色阳性。 Iba1阳性细胞（即巨噬细胞和小胶质细胞）在所有时间点分布在所有区域，但尤其在E80的心室区域聚集。还观察到凋亡形态（在E80）和TUNEL阳性细胞（即，包含DNA片段;在E50和E80）。在E120和E150处，大多数PCNA阳性细胞位于心室区，而在E120处的I-II层以外的所有层中，NeuN阳性细胞均显着。 GFAP免疫反应性主要在白质中具有精细过程的细胞中检测到。在E120或E150均未检测到凋亡或TUNEL阳性细胞。这些结果表明，食蟹猕猴的胎儿大脑在妊娠中期（即E50至E80）发生增殖，迁移和神经细胞死亡，而在妊娠中期之后发生神经细胞的分化和成熟（E80）。

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