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首页> 外文期刊>Nucleic Acids Research >The genome of Streptococcus pneumoniae is organized in topology-reacting gene clusters
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The genome of Streptococcus pneumoniae is organized in topology-reacting gene clusters

机译：肺炎链球菌的基因组组织在拓扑反应基因簇中

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摘要

The transcriptional response of Streptococcus pneumoniae was examined after exposure to the GyrB-inhibitor novobiocin. Topoisomer distributions of an internal plasmid confirmed DNA relaxation and recovery of the native level of supercoiling at low novobiocin concentrations. This was due to the up-regulation of DNA gyrase and the down-regulation of topoisomerases I and IV. In addition, >13% of the genome exhibited relaxation-dependent transcription. The majority of the responsive genes (>68%) fell into 15 physical clusters (14.6-85.6 kb) that underwent coordinated regulation, independently of operon organization. These genomic clusters correlated with AT content and codon composition, showing the chromosome to be organized into topology-reacting gene clusters that respond to DNA supercoiling. In particular, down-regulated clusters were flanked by 11-40 kb AT-rich zones that might have a putative structural function. This is the first case where genes responding to changes in the level of supercoiling in a coordinated manner were found organized as functional clusters. Such an organization revealed DNA supercoiling as a general feature that controls gene expression superimposed on other kinds of more specific regulatory mechanisms.
机译：暴露于GyrB抑制剂新霉素后检查肺炎链球菌的转录反应。内部质粒的拓扑异构体分布证实了在新霉素浓度较低时DNA松弛并恢复了超螺旋的天然水平。这是由于DNA促旋酶的上调和拓扑异构酶I和IV的下调。另外，＞ 13％的基因组表现出松弛依赖性转录。大多数响应基因（> 68％）分为15个物理簇（14.6-85.6 kb），这些簇受到协调调控，独立于操纵子的组织。这些基因组簇与AT含量和密码子组成相关，表明染色体被组织成对DNA超螺旋反应的拓扑反应基因簇。特别是，下调的簇两侧有11-40 kb富含AT的区域，这些区域可能具有假定的结构功能。这是第一个发现以协调方式响应超螺旋水平变化的基因被组织为功能簇的情况。这样的组织揭示了DNA超螺旋作为控制基因表达的一般特征，该基因叠加在其他种类的更具体的调节机制上。

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