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Early detection of bacteria using SPR imaging and event counting: experiments with Listeria monocytogenes and Listeria innocua

机译:使用SPR成像和事件计数的细菌的早期检测:用李斯特菌单核细胞增生和Histeria Innocua进行实验

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摘要

Foodborne pathogens are of significant concern in the agrifood industry and the development of associated rapid detection and identification methods are of major importance. This paper describes the novel use of resolution-optimized prism-based surface plasmon resonance imaging (RO-SPRI) and data processing for the detection of the foodborne pathogens Listeria monocytogenes and Listeria innocua. With an imaging spatial resolution on the order of individual bacteria (2.7 +/- 0.5 m x 7.9 +/- 0.6 m) over a field of view 1.5 mm(2), the RO-SPRI system enabled accurate counting of individual bacteria on the sensor surface. Using this system, we demonstrate the detection of two species of Listeria at an initial concentration of 2 x 10(2) CFU mL(-1) in less than 7 hours. The surface density of bacteria at the point of positive detection was 15 +/- 4 bacteria per mm(2). Our approach offers great potential for the development of fast specific detection systems based on affinity monitoring.
机译:食源性病原体在农业粮食工业中具有重要关注,相关的快速检测和鉴定方法的发展具有重要意义。 本文介绍了分辨率优化棱柱的表面等离子体共振成像(RO-SPRI)和用于检测食品载体的数据处理的新颖使用,以及检测食品毒素李斯特菌和李斯特里亚Innocua。 在1.5mm(2)(2)的视野下,各个细菌(2.7 +/- 0.5 mx 7.9 + 0.6 m)的成像空间分辨率,RO-SPRI系统能够精确计数传感器上的个体细菌 表面。 使用该系统,我们证明在少于7小时的初始浓度为2×10(2)CFU mL(-1)的初始浓度下检测两种李斯特菌。 阳性检测点处的细菌的表面密度为每毫米(2)的15 +/- 4细菌。 我们的方法提供了基于亲和监测的快速特定检测系统的潜力。

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  • 来源
    《RSC Advances》 |2019年第27期|共7页
  • 作者单位

    Univ Grenoble Alpes CNRS CEA INAC SyMMES F-38000 Grenoble France;

    Univ Grenoble Alpes CNRS CEA INAC SyMMES F-38000 Grenoble France;

    Prestodiag 1 Mail Prof Georges Mathe F-94800 Villejuif France;

    Univ Grenoble Alpes CNRS CEA INAC SyMMES F-38000 Grenoble France;

    Univ Grenoble Alpes CNRS CEA INAC SyMMES F-38000 Grenoble France;

    Univ Sherbrooke CNRS UMI 3463 Lab Nanotechnol Nanosyst LN2 UGA 3000 Blvd Univ Sherbrooke PQ J1K OA5 Canada;

    Univ Sherbrooke CNRS UMI 3463 Lab Nanotechnol Nanosyst LN2 UGA 3000 Blvd Univ Sherbrooke PQ J1K OA5 Canada;

    Univ Grenoble Alpes CNRS CEA INAC SyMMES F-38000 Grenoble France;

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  • 正文语种 eng
  • 中图分类 化学;
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