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首页> 外文期刊>Acta biomaterialia >Targeted delivery of mannan-coated superparamagnetic iron oxide nanoparticles to antigen-presenting cells for magnetic resonance-based diagnosis of metastatic lymph nodes in vivo.
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Targeted delivery of mannan-coated superparamagnetic iron oxide nanoparticles to antigen-presenting cells for magnetic resonance-based diagnosis of metastatic lymph nodes in vivo.

机译：将甘露聚糖包衣的超顺磁性氧化铁纳米颗粒靶向递送至抗原呈递细胞，用于基于磁共振的体内转移性淋巴结诊断。

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Metastatic lymph nodes (LN) originate from primary cancer cells that metastasize to the lymphatic system. It is difficult to non-invasively discriminate between metastatic LN and normal LN because of their similarities in size and shape. Magnetic resonance (MR) contrast agents are widely utilized to enhance the image contrast among different tissues. Currently available dextran-based contrast agents are non-specifically internalized by macrophages. Therefore, the aim of this study was to develop mannan-coated superparamagnetic iron oxide nanoparticles (mannan-SPION) for specific delivery to immune cells in LN by receptor-mediated endocytosis for facilitated uptake in the target cells and faster acquisition of MR images. Mannan is a water soluble polysaccharide with a high content of D-mannose residues that can be recognized by mannose receptors on activated macrophages and dendritic cells. Mannan-SPION are proven to be suitable for MRI due to their small size, excellent aqueous stability, and lower cytotoxicity. Mannan-SPION are taken up by antigen-presenting cells such as macrophages and dendritic cells, which could be confirmed by Prussian blue and fluorescent staining. In addition, mannan-SPION exhibit enhanced delivery efficiency in targeting macrophages in LN in vivo compared with polyvinyl alcohol (PVA)-SPION. More specifically, the enhancement of MRI of LN by mannan-SPION increased dramatically during the earlier stages after intravenous injection, compared with PVA-SPION as a control, which indicates the potential for successful and early detection of metastastatic LN.
机译：转移性淋巴结（LN）起源于转移到淋巴系统的原发癌细胞。由于转移性淋巴结和正常淋巴结的大小和形状相似，因此很难以非侵入性的方式对其进行区分。磁共振（MR）造影剂被广泛用于增强不同组织之间的图像对比度。当前可用的基于葡聚糖的造影剂是非特异性地被巨噬细胞内化的。因此，本研究的目的是开发通过受体介导的内吞作用将甘露聚糖包被的超顺磁性氧化铁纳米颗粒（mannan-SPION）特异性递送至LN中的免疫细胞，以促进靶细胞的摄取并更快地采集MR图像。甘露聚糖是具有高含量D-甘露糖残基的水溶性多糖，其可以被活化的巨噬细胞和树突状细胞上的甘露糖受体识别。事实证明，Mannan-SPION具有体积小，出色的水稳定性和较低的细胞毒性，因此适用于MRI。甘露聚糖-SPION被巨噬细胞和树突状细胞等抗原呈递细胞吸收，这可以通过普鲁士蓝和荧光染色来证实。此外，与聚乙烯醇（PVA）-SPION相比，甘露聚糖-SPION在体内靶向LN巨噬细胞时显示出更高的递送效率。更具体地，与作为对照的PVA-SPION相比，在静脉内注射后的早期，甘露聚糖-SPION对LN的MRI增强显着增加，这表明了成功和早期发现转移性LN的潜力。

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