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首页> 外文期刊>Biofabrication >Double-layer perfusable collagen microtube device for heterogeneous cell culture
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Double-layer perfusable collagen microtube device for heterogeneous cell culture

机译：用于异质细胞培养的双层灌注胶原微管器件

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摘要

In vitro perfusable 3D tissue models mimic in vivo tissues and have several benefits in drug testing. However, processes used to fabricate these models often tend to be complicated. Here, we present a double-layer perfusable collagen tube device for multilayered in vitro 3D cell culture. The device is simply made by the repetition of a molding process. The thicknesses of the collagen layers in the tube device can be flexibly designed, and heterogeneous cell types can be co-cultured in/on each collagen layer. Moreover, while our collagen tube is directly attached to silicone tubes, the collagen tube can easily be connected to an external pump system for perfusion culture. We fabricated six different sizes of collagen devices (inner diameter approximately 300-1000 mu m) using different molds, and successfully controlled the coefficients of variation to be below 5% for the diameters of each layer for all six device sizes. The device is strong enough to manipulate with tweezers, and can remain stable for more than 3 months in a medium. For the cell culture, we successfully and correctly encapsulated cells in the layer shape at the desired position, and confirmed cell migration. Using the perfusion culture, we demonstrated that the alignments of the HUVEC actin filaments become parallel to the flow direction. We believe that our device could advance the easy fabrication of various tissue models (that is, models mimicking in vivo tissues). In particular, the device could help fabricate vascularized tissue models, and contribute to the development of pharmacokinetic testing platforms and regenerative medicine.
机译：体外灌注3D组织模型在体内组织中模拟，并在药物检测中具有几个好处。然而，用于制造这些模型的过程通常往往是复杂的。在这里，我们提出了一种用于多层体外3D细胞培养的双层灌注胶原管装置。该装置简单地通过重复模制过程来制造。管装置中的胶原层的厚度可以灵活地设计，并且可以在每个胶原层上/在每个胶原层中共培养异质细胞类型。此外，在我们的胶原管直接连接到硅树脂管中，胶原蛋白可以容易地连接到外部泵系统以进行灌注培养。我们使用不同的模具制造了六种不同尺寸的胶原蛋白装置（内径约300-1000μm），并成功地控制了所有六个设备尺寸的每层直径的5％低于5％。该装置足以用镊子操纵，并且在媒体中可以保持稳定超过3个月。对于细胞培养，我们在期望位置成功和正确地封装了层形状的细胞，并确认了细胞迁移。使用灌注培养，我们证明HUVEC肌动蛋白长丝的比对与流动方向平行。我们认为，我们的设备可以提高各种组织模型的易于制造（即，在体内组织中模拟的模型）。特别地，该装置可以帮助制造血管化的组织模型，并有助于制定药代动力学检测平台和再生医学。

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