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Live imaging of nervous system development and function using light-sheet microscopy

机译:使用光纸显微镜的神经系统开发和功能的实时成像

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In vivo imaging applications typically require carefully balancing conflicting parameters. Often it is necessary to achieve high imaging speed, low photo-bleaching, and photo-toxicity, good three-dimensional resolution, high signal-to-noise ratio, and excellent physical coverage at the same time. Light-sheet microscopy provides good performance in all of these categories, and is thus emerging as a particularly powerful live imaging method for the life sciences. We see an outstanding potential for applying light-sheet microscopy to the study of development and function of the early nervous system in vertebrates and higher invertebrates. Here, we review state-of-the-art approaches to live imaging of early development, and show how the unique capabilities of light-sheet microscopy can further advance our understanding of the development and function of the nervous system. We discuss key considerations in the design of light-sheet microscopy experiments, including sample preparation and fluorescent marker strategies, and provide an outlook for future directions in the field. Mol. Reprod. Dev. 82: 605-618, 2015. (c) 2013 Wiley Periodicals, Inc.
机译:在Vivo成像应用程序中通常需要仔细平衡冲突参数。通常有必要实现高成像速度,低光漂白和光毒性,良好的三维分辨率,高信噪比和同时出色的物理覆盖。光纸显微镜在所有这些类别中提供了良好的性能,因此旨在作为生命科学的一种特别强大的实时成像方法。我们看到将光纸显微镜应用于脊椎动物和更高无脊椎动物的早期神经系统的发展和功能研究的突出潜力。在这里,我们审查了最先进的早期发展的现场成像方法,并展示了光板显微镜的独特能力如何进一步推进我们对神经系统的发展和功能的理解。我们讨论了光板显微镜实验设计中的关键考虑,包括样品制备和荧光标记策略,并为该领域的未来方向提供了一个前景。摩尔。装。开发。 82:605-618,2015。(c)2013 Wiley期刊,Inc。

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