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Discussion on AFLP Molecular Markers in Piper methysticum and Pepper

机译：胡椒和胡椒中AFLP分子标记的探讨

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摘要

The aim of the research was to discuss the genetic relationships between Piper methysticum, Pepper and other wild species in Pepper genus. DNA was extracted from leaves which belonged to 28 germplasms including 6 materials of P. methysticum, 21 materials of cultivated and wild Pepper, 1 material of Peperomia pellucida belonged to different genus. Premiers with good band-type and high polymorphism and resolution were selected from 64 pairs of primers for AFLP amplification and the clustering analysiswas conducted with MVSP3. 13f software. 191 bands were amplified by 4 pairs of premiers, 189 of which had polymorphism, being 98.6%. 28 germplasms were classified into 6 different groups at the genetic similarity coefficient of 0.52 by silver staining AFLP, in which 6 materials of Piper methysticum were clustered into a single group, indicating that P. methysticum belonged to Pepper family of Pepper genus but were distantly related to the others. The research provided the basis for selecting rootstocksfor P. methysticum graft, molecular identification of P. methysticum and the fingerprint construction of P. methysticum.
机译：该研究的目的是讨论胡椒属胡椒，胡椒和其他野生物种之间的遗传关系。从属于28个种质的叶片中提取DNA，其中包括6个种的P. methysticum，21种的栽培和野生胡椒，1种的Peperomia pellucida属于不同属。从64对AFLP扩增引物中选择具有良好带型和高多态性和分辨率的引物，并用MVSP3进行聚类分析。 13f软件。 191条带被4对总理扩增，其中189条具有多态性，占98.6％。银染AFLP法将28个种质按遗传相似系数0.52分为6个不同的组，其中6个胡椒属材料聚为一类，表明该种属属于胡椒属的胡椒科，但远缘相关。给其他人。该研究为选择体育疫病菌的砧木，进行体育毒理学的分子鉴定和指纹图谱的构建提供了依据。

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