...
首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Multi-target plasmid controls for conventional and real-time PCR-based serotyping of Streptococcus pneumoniae
【24h】

Multi-target plasmid controls for conventional and real-time PCR-based serotyping of Streptococcus pneumoniae

机译:基于常规和实时PCR的血管菌血管型血管型血管型血清血清血清血清

获取原文
获取原文并翻译 | 示例
           

摘要

Background: Serotyping of Streptococcus pneumoniae is an integral part of disease surveillance, with over 92 serotypes characterized to date using traditional serotyping. To identify the most predominant disease causing serotypes, molecular serotyping methods are now increasingly being used, like conventional and real-time multiplex PCR (cmPCR and rmPCR, respectively). Given that cmPCR consists of eight reactions spanning 41 targets, and rnWCR consists of seven triplex reactions, standardizing positive controls for these assays is challenging. As such, a 43-target plasmid for cmPCR (pSpn-CM1) and a 23 target plasmid for rmPCR (pSpn-RM1) were designed and validated.
机译:背景:肺炎链球菌的血清型是疾病监测的一个组成部分,具有超过92种血清型,以迄今使用传统血清型特征。 为了鉴定导致血清型的最主要疾病,现在越来越多地使用分子血清型化方法,例如常规和实时多重PCR(CMPCR和RMPCR)。 鉴于CMPCR由八种靶向41靶的八种反应组成,并且RNWCR由七种三重反复反应组成,标准化这些测定的阳性对照是具有挑战性的。 这样,设计并验证了用于CMPCR(PSPN-CM1)的43靶质粒和用于RMPCR(PSPN-RM1)的23个靶质粒。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号