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首页> 外文期刊>Belgian Journal of Zoology >Activation of the GFRa1/NCAM pathway stimulates Sertoli cell proliferation in vitro
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Activation of the GFRa1/NCAM pathway stimulates Sertoli cell proliferation in vitro

机译：GFRa1 / NCAM途径的激活刺激体外支持细胞的增殖

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摘要

The proliferation and final density of Sertoli cells in the testis are regulated by hormones and local growth factors. Glial cell line-derived neurotrophic factor (GDNF), a distantly related member of the transforming growth factor-P (TGF-(a) over cap) Superfamily, and its receptor components: GFR alpha 1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells. However, the number of the reports is very low, and the signalling pathways have not been well characterized. In the present study, we used purified Sertoli cell cultures from 4- to 5-day-old mouse testis to detect the expression of GDNF, to examine the effect of GDNF on Sertoli cell proliferation and possible signalling pathways mediating such effect. GDNF expression in mouse Sertoli cells was detected at both RNA and protein levels by RT-PCR and immunocytochemistry methods. The expression was up-regulated by FSH. GDNF stimulated the proliferation of Sertoli cells and synergized with FSH to promote the proliferation to a higher level. GFR alpha 1/Ret and GFR alpha 1/ NCAM are two receptor complexes for GDNF. As GFR alpha 1 and NCAM not Ret were expressed in cultured Sertoli cells, we proposed that the stimulatory effect of GDNF on Sertoli cell proliferation was through pathways initiating from the GFR alpha 1/INCAM receptor. This proposition was supported by the fact that the stimulatory effect of GDNF was significantly reduced by anti-NCAM antibody.
机译：睾丸支持细胞的增殖和最终密度受激素和局部生长因子的调节。胶质细胞源性神经营养因子（GDNF），转化生长因子P（TGF-（a）帽）的远缘成员，及其受体成分：GFR alpha 1，Ret和神经细胞粘附分子（NCAM最近有报道称其在睾丸中表达并参与未成熟Sertoli细胞的增殖调控。然而，报告的数量非常少，并且信号传导途径还没有得到很好的表征。在本研究中，我们使用4至5天大的小鼠睾丸的纯化Sertoli细胞培养物检测GDNF的表达，以检查GDNF对Sertoli细胞增殖的影响以及可能介导这种作用的信号途径。通过RT-PCR和免疫细胞化学方法在RNA和蛋白质水平检测到小鼠Sertoli细胞中GDNF的表达。该表达被FSH上调。 GDNF刺激Sertoli细胞的增殖，并与FSH协同促进增殖。 GFR alpha 1 / Ret和GFR alpha 1 / NCAM是GDNF的两种受体复合物。由于在培养的支持细胞中表达了GFR alpha 1和NCAM而不是Ret，因此我们提出GDNF对支持细胞的刺激作用是通过GFR alpha 1 / INCAM受体引发的。这一主张得到了以下事实的支持：抗NCAM抗体显着降低了GDNF的刺激作用。

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