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首页> 外文期刊>Journal of mass spectrometry: JMS >A sialylation study of mouse brain gangliosides by MALDI a-TOF and o-TOF mass spectrometry
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A sialylation study of mouse brain gangliosides by MALDI a-TOF and o-TOF mass spectrometry

机译：MALDI A-TOF和O-TOF质谱法的小鼠脑神经节苷脂的唾液酸化研究

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摘要

Matrix-assisted laser desorption/ionization (MALDI) process of sialoglycoconjugates is generally accompanied by different levels of cleavage of sialic acid residues and/or by dehydration, and decarboxylation reactions. Quantitative densitometry of the mouse brain ganglioside (MBG) components separated by high-performance thin layer chromatography (HPTLC) and evidenced by orcinol staining was a basis to verify the ganglioside composition pattern with respect to the relative abundances of individual components in the mixture. A systematic mass spectrometry (MS) sialylation analysis has been carried out to evaluate the feasibility of an axial time-of-flight (a-TOF) MS, equipped with a vacuum MALDI source and an orthogonal-TOF (o-TOF) instrument with an ion source operated at about I mbar of N2Besides, the esterification by one methyl group of the carboxyl group in sialic acid to increase the stability of the ganglioside species for MALDI MS analysis has been tested and the yield of intact ganglioside species and of the neutral loss of water and carbon dioxide estimated. For the sialylation analysis of native ganglioside mixtures the MALDI o-TOF analysis with 6-azo-2-thiothymine/diammonium citrate (ATT/DAC) as a matrix appears as an optimal approach for ganglioside profiling.
机译：基质辅助激光解吸/电离（MALDI）唾液酸核缀合物的方法通常伴随着不同水平的唾液酸残基和/或通过脱水和脱羧酸反应。由高性能薄层色谱（HPTLC）分离的小鼠脑神经胶（MBG）组分的定量密度测定术并通过甲醇染色证明是验证神经节苷脂组合物图案相对于混合物中各个组分的相对丰度的基础。已经进行了系统的质谱（MS）唾液酸化分析，以评估轴向飞行时间（A-TOF）MS的可行性，配备有真空MALDI源和正交-TOF（O-TOF）仪器在N2BESIDE的约I曼巴的离子源，唾液酸中羧基的一个甲基的酯化已经进行了测试，以增加对MALDI MS分析的神经节苷脂物种的稳定性，并且完整神经节苷脂物种和中性的产率损失水和二氧化碳估计。对于天然神经节苷脂的唾液酸化分析混合物，用6-偶氮-2-硫育/二醇（ATT / DAC）作为基质的MALDI O-TOF分析表现为神经节苷脂分析的最佳方法。

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