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首页> 外文期刊>Allergy >Group II subfamily secretory phospholipase A(2) enzymes: expression in chronic rhinosinusitis with and without nasal polyps.
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Group II subfamily secretory phospholipase A(2) enzymes: expression in chronic rhinosinusitis with and without nasal polyps.

机译：第二类亚科分泌型磷脂酶A（2）酶：在有和没有鼻息肉的慢性鼻-鼻窦炎中表达。

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Background: Group II subfamily secretory phospholipases A(2) (sPLA(2)s) are the enzymes that can play a major role in inflammation. However, the presence of group II subfamily sPLA(2)s in human sinonasal mucosa and their roles in chronic rhinosinusitis (CRS) are not well known. The purpose of this study was to investigate the expression of group II subfamily sPLA(2)s in human sinonasal mucosa from controls and CRS patients with and without nasal polyps (NPs) and the regulation of expression by proinflammatory cytokines. Methods: Surgical samples were investigated by means of reverse transcriptase polymerase chain reaction (RT-PCR) for evaluation of group II subfamily sPLA(2)s mRNA expression, and the presence and location of group II subfamily sPLA(2)s-positive cells were analyzed by means of immunohistochemistry. Furthermore, nasal explant culture and quantitative RT-PCR techniques were used to investigate the effect of interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha on group II subfamilysPLA(2)s mRNA production in sinonasal mucosa. Results: Messenger RNA expression of sPLA(2)-IIA, -IID, and -IIE was significantly upregulated in tissues from CRS patients compared with control tissues. Among CRS patients, patients without NPs showed significantly stronger expression in sinonasal mucosa than patients with NPs of sPLA(2)-IIA mRNA, and weaker expression of sPLA(2)-IIE mRNA. Immunohistochemistry revealed enhanced protein expression of type II sPLA(2)s and specific type IIA sPLA(2) in epithelial cells and submucosal glands in samples from CRS patients. Stronger type IIA sPLA(2) protein expression was found in samples from CRS patients without NPs when compared with NPs. Nasal explant culture experiments demonstrated that mRNA expression of sPLA(2)-IIA, -IID, and -IIE was dramatically induced by IL-1beta and TNF-alpha. Conclusions: The expression of some members of group II subfamily of sPLA(2)s is upregulated in CRS and it may result from IL-1beta and TNF-alpha overexpression. Different individual group II subfamily sPLA(2)s may play different roles in the pathogenesis of CRS with and without NPs.
机译：背景：II组亚家族分泌型磷脂酶A（2）（sPLA（2）s）是可以在炎症中起主要作用的酶。但是，尚不清楚人类鼻窦粘膜中是否存在II类亚家族sPLA（2）及其在慢性鼻-鼻窦炎（CRS）中的作用。这项研究的目的是调查在有和没有鼻息肉（NPs）的对照组和CRS患者的人鼻窦粘膜中II类亚家族sPLA（2）的表达以及促炎细胞因子对表达的调节。方法：通过逆转录酶聚合酶链反应（RT-PCR）研究手术标本，以评估II族sPLA（2）s mRNA表达，以及II族sPLA（2）s阳性细胞的存在和定位通过免疫组织化学分析。此外，鼻外植体培养和定量RT-PCR技术用于研究白介素（IL）-1beta和肿瘤坏死因子（TNF）-α对鼻窦粘膜II组亚家族PLA（2）s mRNA产生的影响。结果：与对照组织相比，CRS患者组织中sPLA（2）-IIA，-IID和-IIE的Messenger RNA表达明显上调。在CRS患者中，无NPs的患者鼻窦粘膜中的表达明显高于sPLA（2）-IIA mRNA NPs的患者，而sPLA（2）-IIE mRNA的表达较弱。免疫组织化学显示，CRS患者样本中的上皮细胞和粘膜下腺中II型sPLA（2）和特定的IIA sPLA（2）蛋白表达增强。与NPs相比，在没有NPs的CRS患者样本中发现IIA sPLA（2）型蛋白表达更强。鼻外植体培养实验表明，IL-1beta和TNF-α显着诱导了sPLA（2）-IIA，-IID和-IIE的mRNA表达。结论：CRS中sPLA（2）s第二类亚家族某些成员的表达上调，可能是由于IL-1β和TNF-α的过表达引起的。 II类不同的亚家族sPLA（2）在有和没有NP的CRS发病机理中可能发挥不同的作用。

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