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首页> 外文期刊>Australian Journal of Dairy Technology >Low-cholesterol milk production using cholesterol oxidase from Nocardia erythropolis
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Low-cholesterol milk production using cholesterol oxidase from Nocardia erythropolis

机译：使用来自诺卡氏红藻的胆固醇氧化酶生产低胆固醇牛奶

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摘要

An effective process for reducing milk cholesterol by enzymatic treatment was investigated in this study. Cholesterol oxidase from Nocardia erythropolis was used for this purpose. The target of this investigation was to obtain the optimum operational conditions for bioconversion of milk cholesterol by cholesterol oxidase (COD). The bioconversion process was investigated first, to obtain basic information about the process, and was further optimised by analysing the parameters, including temperature, pH, incubation time and enzyme content, by using the Taguchi Approach, which is a statistical method for optimisation. Also the direct effect of each parameter on cholesterol bioconversion rate has been studied. As a result, the suitable operational conditions were obtained: temperature 32 C, pH 7.5, incubation time of 120 min and enzyme content in milk 0.15 U/mL, which under these conditions, approximately 70% of milk cholesterol was removed. Aust. J. Dairy Technol. 65, 10-14
机译：在这项研究中，研究了通过酶处理降低牛奶胆固醇的有效方法。为此，使用了来自红诺卡氏菌的胆固醇氧化酶。这项研究的目标是获得通过胆固醇氧化酶（COD）生物转化牛奶胆固醇的最佳操作条件。首先对生物转化过程进行了研究，以获得有关该过程的基本信息，并通过使用Taguchi方法（最优化的统计方法）分析了参数（包括温度，pH，孵育时间和酶含量）进行了进一步优化。还研究了每个参数对胆固醇生物转化率的直接影响。结果，获得了合适的操作条件：温度32°C，pH 7.5，孵育时间120分钟，牛奶中的酶含量为0.15 U / mL，在这些条件下，大约70％的牛奶胆固醇被去除。澳洲J.乳业技术。 65、10-14

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