Method for isolation of Escherichia coli 0157:H7 from radish sprouts: a collaborative study

HIROSHI NAKAGAWA; YUKIKO HARA-KUDO; YOUICHI ONOUE

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Method for isolation of Escherichia coli 0157:H7 from radish sprouts: a collaborative study

机译：从萝卜芽中分离大肠杆菌0157：H7的方法：一项合作研究

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Twenty laboratives participated in a collaborative study to evaluate methods for isolating Escherichia coli O157:H7 from radish sprouts. The collaborators attempted to isolate the organism from 25 g of radish sprouts inoculated with 28.3 CFU E. coli O157:H7, using enrichment and plating methods. E. coli O157:H7 could be isolated from most samples after being cultured at 42 deg C for 18 h in a modified EC broth supplemented with novobiocin but not after being cultured at 37 deg C for 6 h in tryptic soy broth.

机译：20名实验室人员参加了一项合作研究，以评估从萝卜芽中分离大肠埃希氏菌O157：H7的方法。合作者尝试使用富集和平板接种方法从接种28.3 CFU大肠杆菌O157：H7的25 g萝卜芽中分离出该生物。在添加了新生霉素的改良EC肉汤中于42℃下培养18小时后，可在大多数样品中分离出O157：H7大肠杆菌，而在胰蛋白酶大豆肉汤中于37℃下培养6小时后，无法从大肠杆菌中分离出O157：H7。

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《Biocontrol Science》 |1999年第1期|共5页
作者
HIROSHI NAKAGAWA; YUKIKO HARA-KUDO; YOUICHI ONOUE;
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原文格式 PDF
正文语种 eng
中图分类临床医学;
关键词
Escherichia coli 0157:H7; radish sprouts; ground beef;

机译：大肠杆菌0157：H7;萝卜芽;碎牛肉;

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1. Method for isolation of Escherichia coli 0157:H7 from radish sprouts: a collaborative study [J] . HIROSHI NAKAGAWA, YUKIKO HARA-KUDO, YOUICHI ONOUE Biocontrol Science . 1999,第1期

机译：从萝卜芽中分离大肠杆菌0157：H7的方法：一项合作研究
2. The Effect of Calcinated Calcium and Chlorine Treatments on Escherichia coli 0157:H7 87-23 Population Reduction in Radish Sprouts [J] . Lilia Fransisca, Bin Zhou, Heekyung Park, Journal of Food Science . 2011,第6期

机译：钙和氯的煅烧处理对萝卜新芽中大肠杆菌0157：H7 87-23种群减少的影响
3. Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts [J] . Tomohiko Fujisawa, Shin Sata, Katsuhiro Aikawa, Applied and Environmental Microbiology . 2000,第7期

机译：含头孢克肟和亚碲酸盐的山梨醇MacConkey培养基的修饰，用于从萝卜芽中分离大肠杆菌O157：H7
4. COMPARISON OF METHODS FOR THE DETECTION OF CULTURABLE AND VBNC ESCHERICHIA COLI 0157:H7 IN COMPLEX DRINKING WATER BIOFILMS [C] . S. A. Wilks, M. S. Giao, C. W. Keevil International Conference on Water Contamination Emergencies . 2013

机译：检测培养和VBNC大肠杆菌0157中的方法的比较：H7复合饮用水生物膜
5. Methods to study the physical locations of lactic acid bacteria and Escherichia coli O157:H7 in fermented sausages. [D] . Lee, Nancy Chunlan. 2003

机译：研究发酵香肠中乳酸菌和大肠杆菌O157：H7的物理位置的方法。
6. Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts [O] . Tomohiko Fujisawa, Shin Sata, Katsuhiro Aikawa, 2000

机译：含头孢克肟和亚碲酸盐的山梨醇MacConkey培养基的修饰用于从萝卜芽中分离大肠杆菌O157：H7
7. Method for Isolation of Escherichia coli O157:H7 from Radish Sprouts: A Collaborative Study. [O] . HIROSHI NAKAGAWA, YUKIKO HARA-KUDO, YOUICHI ONOUE, 1999

机译：从萝卜豆芽中分离大肠杆菌O157：H7的方法：一个协作研究。
8. Searching for a Water Supply Connection in the Cabool, Missouri Disease Outbreakof 'Escherichia coli' 0157:H7 [R] . Geldreich, E. E., Fox, K. R., Goodrich, J. A., 1992

机译：在密苏里州疾病爆发的大肠杆菌'0157：H7中寻找供水连接

Method for isolation of Escherichia coli 0157:H7 from radish sprouts: a collaborative study

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