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首页> 外文期刊>Biocontrol Science and Technology >Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strain
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Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strain

机译：苏云金芽孢杆菌菌株对鳞翅目幼虫有效的新型cry1H基因的克隆与鉴定

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摘要

We characterised a novel holotype cry gene (cry1Hc1) harboured in BN23-5 Bt strain which was isolated from soil samples in Sichuan, China. In this study, the full length of the cry1Hc1 gene was cloned from the strain. The cry1Hc1 gene was inserted into a shuttle vector (pSTK) and expressed in an acrystalliferous mutant Bacillus thuringiensis HD73(-). In this transformant, cry1Hc1 was expressed and diamond-shaped parasporal crystals were formed. The resulting insecticidal activity showed that the Cry1Hc1 protein exhibited high larvicidal activity against larvae of Plutella xyllostella and Ostrinia furnacalis with lethal concentration 50 of 55.21 and 118.44 mu g/ml, respectively. Sequence analysis of the gene was also performed; the Cry1Hc1 protein retained eight conserved regions commonly found in the existing Cry proteins.
机译：我们表征了BN23-5 Bt菌株中包含的一个新的全基因哭泣基因（cry1Hc1），该菌株是从中国四川的土壤样品中分离出来的。在这项研究中，从菌株中克隆了cry1Hc1基因的全长。将cry1Hc1基因插入穿梭载体（pSTK），并在无晶体突变的苏云金芽孢杆菌HD73（-）中表达。在该转化子中，表达了cry1Hc1并形成了菱形的伴孢晶体。产生的杀虫活性表明，Cry1Hc1蛋白对小菜蛾（Plutella xyllostella）和糠Ost（Ostrinia furnacalis）的幼虫表现出较高的杀幼虫活性，致死浓度50分别为55.21和118.44μg/ ml。还对该基因进行了序列分析。 Cry1Hc1蛋白保留了现有Cry蛋白中常见的八个保守区。

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