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首页> 外文期刊>International Dairy Journal >Liquid chromatographic method for the determination of lutein in milk and pediatric formulas
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Liquid chromatographic method for the determination of lutein in milk and pediatric formulas

机译：液相色谱法测定牛奶和儿科配方食品中的叶黄素

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摘要

A simple and rapid method has been developed for routine compliance testing of lutein in pediatric formulas. Following a mild saponification procedure (70^oC for 10min) demonstrated to be benign to lutein, lipophilic components were partitioned by a single extraction into hexane:diisopropyl ether (75:25 v/v) and concentrated in mobile phase. Baseline chromatographic separation of lutein and zeaxanthin (internal standard) was achieved using a C"3"0 column and isocratic elution with methanol:dichloromethane (70:30 v/v). Lutein concentration was quantitated against zeaxanthin, which corrected for incomplete extraction recovery. Performance parameters assessed included recovery (101-108%) and repeatability (2.2% RSD). The method was applied to the analysis of lutein-supplemented pediatric formulas, unsupplemented milk powders, and bovine and human milk.
机译：已经开发出一种简单而快速的方法，用于儿科配方中叶黄素的常规顺应性测试。在温和的皂化步骤（70°C，10分钟）证明对叶黄素无害后，通过单次萃取将亲脂性成分分配到己烷：二异丙醚（75:25 v / v）中，并浓缩在流动相中。叶黄素和玉米黄质（内标）的基线色谱分离采用C“ 3” 0色谱柱进行，并用甲醇：二氯甲烷（70:30 v / v）等度洗脱。对玉米黄质定量叶黄素浓度，校正了不完全的提取回收率。评估的性能参数包括回收率（101-108％）和重复性（2.2％RSD）。该方法可用于分析补充叶黄素的儿科配方，未补充的奶粉以及牛和人乳。

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