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Java web tools for PCR, in silico PCR, and oligonucleotide assembly and analysis.

机译:用于PCR,计算机PCR以及寡核苷酸组装和分析的Java网络工具。

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The polymerase chain reaction is fundamental to molecular biology and is the most important practical molecular technique for the research laboratory. We have developed and tested efficient tools for PCR primer and probe design, which also predict oligonucleotide properties based on experimental studies of PCR efficiency. The tools provide comprehensive facilities for designing primers for most PCR applications and their combinations, including standard, multiplex, long-distance, inverse, real-time, unique, group-specific, bisulphite modification assays, Overlap-Extension PCR Multi-Fragment Assembly, as well as a programme to design oligonucleotide sets for long sequence assembly by ligase chain reaction. The in silico PCR primer or probe search includes comprehensive analyses of individual primers and primer pairs. It calculates the melting temperature for standard and degenerate oligonucleotides including LNA and other modifications, provides analyses for a set of primers with prediction of oligonucleotide properties, dimer and G-quadruplex detection, linguistic complexity, and provides a dilution and resuspension calculator.
机译:聚合酶链反应是分子生物学的基础,是研究实验室最重要的实用分子技术。我们已经开发并测试了用于PCR引物和探针设计的有效工具,这些工具还可以根据PCR效率的实验研究预测寡核苷酸的特性。这些工具可为大多数PCR应用及其组合设计引物提供全面的工具,包括标准,多重,长距离,逆向,实时,独特,特定于组的亚硫酸氢盐修饰测定,重叠扩展PCR多片段组装,以及设计用于通过连接酶链反应进行长序列组装的寡核苷酸集的程序。电子PCR PCR引物或探针搜索包括对单个引物和引物对的全面分析。它可以计算标准寡核苷酸和简并寡核苷酸(包括LNA和其他修饰)的解链温度,提供对一组引物的分析,以预测寡核苷酸特性,二聚体和G-四链体检测,语言复杂性,并提供稀释和重悬计算器。

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