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首页> 外文期刊>Methods in cell science: an official journal of the Society for In Vitro Biology >Pulsed-field gel electrophoresis as an epidemiologic tool for enterococci and streptococci
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Pulsed-field gel electrophoresis as an epidemiologic tool for enterococci and streptococci

机译：脉冲场凝胶电泳作为肠球菌和链球菌的流行病学工具

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摘要

Enterococci (Enterococcus faecium and Enterococcus faecalis) and streptococci such as Streptococcus pyogenes (Group A streptococcus), Streptococcus agalactiae (Group B streptococcus), and Streptococcus pneumoniae are increasing in importance as both hospital-acquired and community pathogens. Emerging resistance and increasing incidence of these organisms has necessitated the analysis of their epidemiologic mechanisms of spread. Pulsed-field gel electrophoresis (PFGE) has emerged as the one of the most widely applicable, reproducible, and stable methods to examine strain identity in bacterial organisms. The procedure used in our laboratory for PFGE typing of whole cell DNA digested with SmaI for enterococci, S. pneumoniae, S. pyogenes, and S. agalacatiae is presented. Issues regarding interpretation are also reviewed and discussed.
机译：肠球菌（粪肠球菌和粪肠球菌）和链球菌如化脓性链球菌（A族链球菌），无乳链球菌（B族链球菌）和肺炎链球菌在医院获得和社区致病菌中的重要性都在增加。这些生物的新出现的抗药性和发病率的增加已需要分析其传播的流行病学机制。脉冲场凝胶电泳（PFGE）已经成为检查细菌生物体中菌株身份的最广泛应用，可重现和稳定的方法之一。介绍了在我们实验室中用于用SmaI消化的全球DNA的PFGE分型的方法，用于肠球菌，肺炎链球菌，化脓性链球菌和琼脂链球菌。有关解释的问题也将进行审查和讨论。

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