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A simple microtiter plate screening assay for bacterial invasion or adherence

机译:用于细菌侵入或粘附的简单的微量滴定板筛选测定

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摘要

Many bacteriologic studies, including cellular invasion and adherence assays, require enumeration of viable organisms or colony forming units. When attempting to screen large numbers of clinical or environmental isolates or laboratory-derived mutants for differences in invasion or adherence phenotype, standard plating methods can be cumbersome and severely limit the number of organisms or conditions which can be tested. As a potential alternative, we describe a simple, rapid and inexpensive soft agar-based technique for semi-quantitative determination of bacterial colony counts directly within the wells of a 96-well microtiter plate.
机译:许多细菌学研究,包括细胞侵袭和粘附试验,都需要列举活生物体或菌落形成单位。当尝试筛选大量临床或环境分离株或实验室衍生的突变体的侵袭或粘附表型差异时,标准的铺板方法可能很麻烦,并严重限制了可以测试的生物或条件的数量。作为一种潜在的替代方法,我们描述了一种简单,快速且便宜的基于软琼脂的技术,可直接在96孔微量滴定板孔内半定量测定细菌菌落数。

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