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首页> 外文期刊>Mycologia >Genome-wide in silico identification of GPI proteins in Mycosphaerella fijiensis and transcriptional analysis of two GPI-anchored beta-1,3-glucanosyltransferases
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Genome-wide in silico identification of GPI proteins in Mycosphaerella fijiensis and transcriptional analysis of two GPI-anchored beta-1,3-glucanosyltransferases

机译：全基因组的斐济支原体中GPI蛋白的计算机鉴定和两个GPI锚定的β-1,3-葡糖基转移酶的转录分析

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摘要

The hemibiotrophic fungus Mycosphaerella fijiensis is the causal agent of black Sigatoka (BS), the most devastating foliar disease in banana (Musa spp.) worldwide. Little is known about genes that are important during M. fijiensis-Musa sp. interaction. The fungal cell wall is an attractive area of study because it is essential for maintenance of cellular homeostasis and it is the most external structure in the fungal cell and therefore mediates the interaction of the pathogen with the host. In this manuscript we describe the in silico identification of glycosyl phosphatidylinositol-protein (GPI) family in M. fijiensis, and the analysis of two beta-1,3-glucanosyltransferases (Gas), selected by homology with fungal pathogenicity factors. Potential roles in pathogenesis were evaluated through analyzing expression during different stages of black Sigatoka disease, comparing expression data with BS symptoms and fungal biomass inside leaves. Real-time quantitative RT-PCR showed nearly constant expression of MfGAS1 with slightly increases (about threefold) in conidia and at specknecrotrophic stage during banana-pathogen interaction. Conversely, MfGAS2 expression was increased during biotrophy (about seven times) and reached a maximum at speck (about 23 times) followed by a progressive decrease in next stages, suggesting an active role in M. fijiensis pathogenesis.
机译：半生营养真菌斐济支原体是黑色Sigatoka（BS）的病原体，它是全世界香蕉（Musa spp。）中最具破坏性的叶面疾病。关于斐济分支杆菌-Musa sp。期间重要基因的了解甚少。相互作用。真菌细胞壁是一个有吸引力的研究领域，因为它对于维持细胞稳态是必不可少的，并且它是真菌细胞中最外部的结构，因此可以介导病原体与宿主之间的相互作用。在这份手稿中，我们描述了斐济分枝杆菌中糖基磷脂酰肌醇蛋白（GPI）家族的计算机鉴定，并分析了两个与真菌致病性因子同源的β-1,3-葡糖基转移酶（Gas）。通过分析黑色Sigatoka病不同阶段的表达，将表达数据与BS症状和叶片内部的真菌生物量进行比较，评估了在发病机理中的潜在作用。实时定量RT-PCR显示香蕉和病原体相互作用过程中，分生孢子中和散养期的MfGAS1几乎恒定表达，并略有增加（约三倍）。相反，MfGAS2表达在生物营养期间增加（约7倍），并在斑点处达到最大值（约23倍），随后在下一阶段逐渐降低，表明在斐济分支杆菌的发病机理中起积极作用。

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