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首页> 外文期刊>Cell metabolism >In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis.
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In vivo mapping of hydrogen peroxide and oxidized glutathione reveals chemical and regional specificity of redox homeostasis.

机译：体内过氧化氢和氧化型谷胱甘肽的图谱揭示了氧化还原稳态的化学和区域特异性。

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The glutathione redox couple (GSH/GSSG) and hydrogen peroxide (H(2)O(2)) are central to redox homeostasis and redox signaling, yet their distribution within an organism is difficult to measure. Using genetically encoded redox probes in Drosophila, we establish quantitative in vivo mapping of the glutathione redox potential (E(GSH)) and H(2)O(2) in defined subcellular compartments (cytosol and mitochondria) across the whole animal during development and aging. A chemical strategy to trap the in vivo redox state of the transgenic biosensor during specimen dissection and fixation expands the scope of fluorescence redox imaging to include the deep tissues of the adult fly. We find that development and aging are associated with redox changes that are distinctly redox couple-, subcellular compartment-, and tissue-specific. Midgut enterocytes are identified as prominent sites of age-dependent cytosolic H(2)O(2) accumulation. A longer life span correlated with increased formation of oxidants in the gut, rather than a decrease.
机译：谷胱甘肽氧化还原对（GSH / GSSG）和过氧化氢（H（2）O（2））是氧化还原稳态和氧化还原信号的中心，但它们在生物体内的分布很难测量。在果蝇中使用遗传编码的氧化还原探针，我们在整个发育过程中，在整个动物中建立了确定的亚细胞区室（细胞质和线粒体）中的谷胱甘肽氧化还原电位（E（GSH））和H（2）O（2）的定量体内作图。老化。一种在标本解剖和固定过程中捕获转基因生物传感器体内氧化还原状态的化学策略，将荧光氧化还原成像的范围扩大到了成年蝇的深层组织。我们发现发育和衰老与氧化还原变化有关，氧化还原变化明显是氧化还原对，亚细胞区室和组织特异性的。中肠肠上皮细胞被确定为年龄依赖的胞质H（2）O（2）积累的重要站点。更长的寿命与肠道中氧化剂形成的增加有关，而不是减少。

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