Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction.

Casebolt DB; Qian Biao; Stephensen CB; Qian B

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Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction.

机译：聚合酶链反应检测肠道型小鼠肝炎病毒粪便排泄。

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A reverse transcription and polymerase chain reaction (PCR) method was developed and used to detect enterotropic mouse hepatitis virus (MHV) in faeces. A nested set of primers for PCR was located in the polymerase region of the MHV genome. The assaywas used to follow the time course of enterotropic MHV excretion in mice after oronasal inoculation with the virus. The assay detected MHV genome excretion in all mice beginning on day 3 through day 21 after inoculation. No MHV genome was detected in inoculated mice on or after day 27 following inoculation. It is concluded that reverse transcription and PCR are useful for enterotropic MHV detection.

机译：开发了一种逆转录和聚合酶链反应（PCR）方法，用于检测粪便中的肠道型小鼠肝炎病毒（MHV）。嵌套的一组PCR引物位于MHV基因组的聚合酶区域中。该测定用于追踪经口鼻接种病毒后小鼠的促肠型MHV排泄的时间过程。该测定法从接种后的第3天到第21天检测了所有小鼠的MHV基因组排泄。在接种后第27天或之后，在接种的小鼠中未检测到MHV基因组。结论是反转录和PCR可用于促肠型MHV检测。

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来源
《Laboratory Animal Science》 |1997年第1期|共5页
作者
Casebolt DB; Qian Biao; Stephensen CB; Qian B;
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正文语种 eng
中图分类动物学实验（实验动物学）、动物学技术;
关键词
detection; faeces; assays; diagnostic techniques; laboratory animals; polymerase chain reaction; viral diseases; biotechnology; mice; Coronaviridae; Canada;

机译：检测;粪便;化验;诊断技术;实验室动物;聚合酶链反应;病毒性疾病;生物技术;小鼠;冠状病毒科;加拿大;

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1. Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction. [J] . Casebolt DB, Qian Biao, Stephensen CB, Laboratory Animal Science . 1997,第1期

机译：聚合酶链反应检测肠道型小鼠肝炎病毒粪便排泄。
2. Microwave treatment of serum facilitates detection of hepatitis B virus DNA by the polymerase chain reaction. Results of a study in anti-HBe positive chronic hepatitis B. [J] . Costa J, Lopez Labrador FX, Sanchez Tapias JM, Journal of Hepatology: The Journal of the European Association for the Study of the Liver . 1995,第1期

机译：血清的微波处理有助于通过聚合酶链反应检测乙型肝炎病毒DNA。抗HBe阳性慢性乙型肝炎的研究结果。
3. Microwave treatment of serum facilitates detection of hepatitis B virus DNA by the polymerase chain reaction. Results of a study in anti-HBe positive chronic hepatitis B. [J] . Costa J, Lopez Labrador FX, Sanchez Tapias JM, Journal of Hepatology: The Journal of the European Association for the Study of the Liver . 1995,第1期

机译：血清的微波处理有助于通过聚合酶链反应检测乙型肝炎病毒DNA。抗HBe阳性慢性乙型肝炎的研究结果。
4. Design of Integrated Instrument for Polymerase Chain Reaction, Biochip Hybridization and Fluorescence Detection Part Two: A New Biochip to Detection Hepatitis B Virus Mutation [C] . Liu quanjun, Zhu Jijun, Zhao Wei, Instrumentation Science and Technology Vol.2 . 2002

机译：聚合酶链反应，生物芯片杂交和荧光检测集成仪器的设计第二部分：检测乙型肝炎病毒突变的新型生物芯片
5. Development of a rapid detection method of Salmonella spp. on chicken skin by real-time polymerase chain reaction. [D] . Carter, Melvin. 2008

机译：沙门氏菌快速检测方法的开发。通过实时聚合酶链反应在鸡皮上
6. Detection of human immunodeficiency virus hepatitis B virus and hepatitis C virus in donor eyes using polymerase chain reaction. [O] . J Shimazaki, K Tsubota, M Sawa, 1994

机译：使用聚合酶链反应检测供体眼睛中的人类免疫缺陷病毒乙型肝炎病毒和丙型肝炎病毒。
7. Application of Nested Polymerase Chain Reaction to Detection of Mouse Hepatitis Virus in Fecal. Specimens during a Natural Outbreak in an Immunodeficient Mouse Colony. [O] . Yasuko K. YAMADA, Mikiko YABE, Kazuhiro TAKIMOTO, 1998

机译：巢式聚合酶链反应在粪便中检测小鼠肝炎病毒的应用。在免疫缺陷的小鼠殖民地的自然爆发过程中标本。

Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction.

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