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首页> 外文期刊>Research communications in molecular pathology and pharmacology >Enhanced Ca2+ sensitization of the bronchial smooth muscle contraction in antigen-induced airway hyperresponsive rats.
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Enhanced Ca2+ sensitization of the bronchial smooth muscle contraction in antigen-induced airway hyperresponsive rats.

机译：抗原诱导的气道高反应性大鼠中支气管平滑肌收缩的Ca2 +增敏作用。

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摘要

To investigate the alteration in acetylcholine (ACh)-induced increase in Ca2+ sensitization of bronchial smooth muscle contraction concurrent with the airway hyperresponsiveness (AHR), the ACh-induced increases in cytosolic Ca2+ ([Ca2+]) level and contractile response were simultaneously determined by using Fura-2 loaded bronchial smooth muscle. The left main bronchi were isolated from AHR rats which were sensitized and repeatedly challenged with DNP-Ascaris antigen. The tissue ring preparations were incubated in loading solution containing 10 microM Fura-2AM for 3 hr at room temperature. Then the isometrical contraction and [Ca2+]i (F340/F380) were monitored. Although the ACh (10(-3) M)-induced contractile response in AHR group (322 +/- 60 % of 60 mM K+ induced contraction) was significantly greater than that in control animals (173 +/- 15 %, p<0.05), the ACh (10(-3) M)-induced increase in [Ca2+]i was without significant difference between the two groups (128 +/- 15 and 171 +/- 29% of 60 mM K+ -induced increase in [Ca2+]i, respectively). These findings suggest that an augmentation of ACh-induced Ca2+ sensitization may occur in bronchial smooth muscle of the rats with antigen-induced AHR.
机译：为了研究乙酰胆碱（ACh）引起的支气管平滑肌收缩的Ca2 +敏感性增高与气道高反应性（AHR）的变化，同时测定ACh诱导的胞浆Ca2 +（[Ca2 +]）水平和收缩反应的增加使用Fura-2加载支气管平滑肌。从AHR大鼠中分离出左主支气管，将其敏化并反复用DNP-Ascaris抗原攻击。在室温下，将组织环制剂在含有10 microM Fura-2AM的上样溶液中孵育3小时。然后监测等距收缩和[Ca2 +] i（F340 / F380）。尽管AHR组（10（-3）M）引起的ACh（10（-3）M）诱导的收缩反应（60 mM K +诱导的收缩的322 +/- 60％）显着大于对照动物（173 +/- 15％，p < 0.05），ACh（10（-3）M）诱导的[Ca2 +] i的增加在两组之间没有显着差异（60 mM K +引起的Ca + +的增加的128 +/- 15和171 +/- 29％）。 [Ca2 +] i）。这些发现表明，在抗原诱导的AHR大鼠的支气管平滑肌中可能发生ACh诱导的Ca 2+增敏。

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