...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Sexually transmitted diseases >Neisseria gonorrhoeae MS11mkC opacity protein expression in vitro and during human volunteer infectivity studies.
【24h】

Neisseria gonorrhoeae MS11mkC opacity protein expression in vitro and during human volunteer infectivity studies.

机译：淋病奈瑟氏球菌MS11mkC不透明蛋白在体外和人类志愿者感染性研究中的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND AND OBJECTIVES: Neisseria gonorrhoeae MS11mkC harbors 11 independently expressed opacity (Opa) protein genes with distinct in vitro expression frequencies. In experimental infections in which human male volunteers were inoculated with transparent (Opa), piliated (P+) strains, the authors associate onset of symptoms with recovery of opaque (Opa+) gonococci. GOALS: In vitro and recovered (Opa) protein expression rates were compared to determine if the human host influences Opa expression. STUDY DESIGN: Opa expression was determined using Western immunoblot analysis; Opa sizes were determined using a scanning densitometer. RESULTS: Seven of 10 Opa proteins were identified in gonococci recovered from all of the volunteers at frequencies consistent with in vitro results (Opa C, 29.5 kDa; Opa K, 30 kDa; Opa G, 31 kDa; Opa I, 32 kDa; Opa J, 33 kDa; Opa D, 34 kDa; and Opa H, 37 kDa) (P > or = 0.01, Fisher exact test). Opa B (30.5 kDa) was identified at lower than expected frequencies, whereas Opa E (31.2) and F (31.5) were identified at higher' than expected frequencies. When recovered gonococci were reanalyzed for in vitro expression frequencies, they were consistent with preinfection frequencies. CONCLUSIONS: The host may influence the prevalence of some Opa proteins.
机译：背景与目的：淋病奈瑟氏球菌MS11mkC包含11个独立表达的不透明（Opa）蛋白基因，具有不同的体外表达频率。在人类男性志愿者接种透明（Opa），有毛（P +）菌株的实验感染中，作者将症状的发作与不透明（Opa +）淋球菌的恢复联系起来。目标：比较体外和恢复的（Opa）蛋白表达速率，以确定人宿主是否影响Opa表达。研究设计：使用Western免疫印迹分析确定Opa表达;使用扫描密度计确定Opa大小。结果：从所有志愿者中回收的淋球菌中鉴定出10种Opa蛋白中的7种，频率与体外结果一致（Opa C，29.5 kDa; Opa K，30 kDa; Opa G，31 kDa; Opa I，32 kDa; Opa J，33 kDa; Opa D，34 kDa; Opa H，37 kDa）（P>或= 0.01，Fisher精确检验）。 Opa B（30.5 kDa）的频率低于预期频率，而Opa E（31.2）和F（31.5）的频率高于预期频率。重新分析回收的淋球菌的体外表达频率时，它们与感染前频率一致。结论：宿主可能影响某些Opa蛋白的流行。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号